肾癌各期患者调节性T细胞的表达

目的检测并比较Ⅰ期~Ⅲ期肾癌患者体内调节性T细胞(Tregs)的分布变化并探索可能的趋化机制。方法收集30例肾癌患者血液及组织标本,其中Ⅰ期~Ⅲ期患者各10例;另选取同期6例非肾癌患者标本予以对照。应用流式细胞术及免疫组化检测外周血及组织的CD4+CD25+Foxp3+Tregs表达;应用RealtimePCR检测组织中CCR4/CCL22转录水平。结果肾癌患者外周血CD25+Foxp3+Tregs和肾癌组织中Foxp3经定量统计分析显示,Ⅰ期~Ⅲ期均高于非肿瘤对照组,差异均有统计学意义(t分别=5.33、8.30、6.14、9.85、8.29、20.14,P均<0.05),且随肿瘤分期增高,Tregs和Foxp3水平亦呈相应上升趋势,差异均有统计学意义(t分别=2.24、8.29、2.45、5.51,P均<0.05)。实时定量PCR结果,显示肾癌组织局部趋化因子CCR4及其配体CCL22转录水平较非肿瘤对照组明显增加,差异均有统计学意义(t分别=5.68、8.97、6.68、12.98、13.28、11.98,P均<0.05)。结论肾癌患者外周血及肿瘤组织中Tregs含量较非肿瘤患者升高,从而抑制机体抗肿瘤免疫功能。肿瘤组织高表达CCL22,从而趋化Tregs至肿瘤组织内。

Distribution and chemotaxis mechanism of regulatory T cells in patients with kidney carcinoma

Objective To investigate the distribution and chemotaxis mechanism of regulatory T cells in kidney carcino- ma patients. Methods The blood sample and tissue specimen of 30 kidney carcinoma patients（stage Ⅰ, n=10; stage Ⅱ, n=10; stage Ⅲ,n=10） and non-kidney carcinoma patients were collected. The expression of CD4^＋CD25^＋Foxp3^＋ Tregs in peripheral blood and tissue was analyzed. The expression of CCR4/CCL22 in tissue was detected by real-time PCR. Results The peripheral blood percentage of CD25^＋Foxp3q^＋regs and Foxp3^＋ in kidney carcinoma patients was higher than that of the control group, and increasing with tumor stage （t=5.33,8.30,6.14,9.85,8.29,20.14,P〈0.05）. The tissue expression of Tregs and Foxp3 in kidney carcinoma patients was higher than that of the control group, and increasing with tumor stage （t=2.24,8.29,2.45,5.51 ,P〈0.05）. The tissue expression of CCR4/CCL22 in kidney carcinoma patients was higher than that of the control group （t=5.68,8.97,6.68,12.98,13.28,11.98,P〈0.05）. Conclusions The percentage of Tregs in kid- ney carcinoma patients, which could suppress anti-tumor immune function, were higher than that of the control group both in peripheral blood and tissue. The tumor tissue expressed much more CCL22, as a ligand of CCR4 in Tregs, and attracted them into tumor.