D-半乳糖诱导小鼠肝损伤模型的方法研究

目的探讨不同实验方法条件下，D-半乳糖所致小鼠肝损伤各项相关指标的变化及意义。方法采用4周腹腔注射染毒、急性腹腔注射染毒、急性尾静脉注射染毒的方法，测定不同剂量组小鼠血清中AIJT、AST及肝匀浆中SOD、GSH、MDA，肝细胞活性氧（Ros）水平、细胞凋亡率。结果4周腹腔注射染毒各组各指标与对照相比，差异无统计学意义（P〉n05），模型不成立。急性腹腔注射、急性尾静脉注射各剂量组相关指标变化提示，2种方法均可建立肝损伤模型，但尾静脉注射各组相关指标剂量-效应关系好于腹腔注射各组，灵敏度也更高。结论与其他2种方法相比，急性尾静脉注射D-半乳糖诱导小鼠肝损伤模型，具有染毒特异性高、剂量-效应关系好、指标特异性和灵敏度高等特点，是较理想的肝损伤模型复制方法。

Study on the Methodology of D-galactose Induced Mouse Liver Injury Model

Objective] To investigate the changes of the relevant indicators in the liver injury in mice induced by D-galactose under various experimental conditions. Methods ] D-galactose was delivered into mice by intraperitoneal injection in 4 weeks, acute intrap- eritoneal injection, and acute intravenous injection in tail, respectively. The ALT and AST activities in mouse serum, the SOD, GSH and MDA in liver homogenate and the reactive oxygen species I ROS } level and apoptosis percentages of hepatocytes were meas- ured respectively to evaluate the liver injury. Results] Comparing the group with intraperitoneal injection with control group, the re- lated liver indexes did not show significant differences { P 〉 0.05 ） , indicating that the liver injury model was unsubstantiated. Either acute intraperitoneal or intravenous injection of D-galactose could form liver injury model, revealed by the above mentioned indexes. Intravenous injection was prior to intraperitoneal injection in establishing the mouse liver injury model, with better dose-response re- lationship and higher sensitivity. Conclusion] In contrast to the other 2 experimental methods, intravenous injection of D-galactose is more effective in inducing mouse liver injury with better specificity, sensitivity and dose-response relationship, and is suggested a potent method in coping model of liver injuries.