四君子汤加味防治烫伤后大鼠肠道损伤和细菌移位实验研究

目的　研究中药四君子汤加味对减轻烫伤后肠道损伤的影响。　方法　Wistar大鼠随机分为 3组 :烫伤治疗组 (简称治疗组 )管饲党参、茯苓、白术、甘草、大黄中药煎剂 2ml,2次 d ,烫伤对照组 (简称对照组 )和正常组管饲同量蒸馏水。根据指标设定于伤后不同时相点 ,分别处死大鼠检测肠粘膜和血浆中肿瘤坏死因子 (TNF)、一氧化氮 (NO)、丙二醛 (MDA)、ATP酶活性及肠粘液中S IgA含量 ,观察肠粘膜病理变化 ,行脏器组织细菌培养、血培养。　结果　中药四君子汤加味可明显降低烫伤大鼠血浆和肠粘膜TNF、NO和MDA含量 ,维持肠粘膜细胞分泌S IgA的功能 ,恢复T细胞数量 ,减轻肠粘膜屏障损害 ,减少脏器细菌检出率。　结论　中药四君子汤加味能够减轻肠粘膜损伤 ,防止肠道细菌移位

An experimental study on the prevention and treatment of postburn intestinal injury and bacterial translocation by Sijunzi decoction in scalded rats

Objective To explore the effects of traditional Chinese herbal medicine Sijunzi decoction on amelioration of postburn intestinal injury in scalded rats. Methods One hundred and eighty Wistar rats were randomly divided into 3 groups, i.e. scald and treatment (T), scald control (S) and normal control (C) groups. The rats in T group were gavaged with the decoction consisting of tangshen, tuckahoe, largehead atractylodes rhizome, glycyrrhizia and rhubarb in a dose of 2 ml twice daily, while the rats in C group were just gavaged with the same amount of distilled water. The rats were sacrificed according to the scheduled postburn observation timepoints. The contents of TNF, NO, MDA and ATPase activity in rat plasma and the intestinal mucosa and the S IgA content in the intestinal mucus were determined respectively. The changes in histopathology of intestinal mucosa were observed. The samples from internal organ tissue and blood were obtained for bacterial culture. Results The contents of TNF, NO and MDA in the intestinal mucosa tissue and the rat plasma in scalded rats were lowered significantly by Sijunzi decoction. Furthermore, S IgA secretion from intestinal mucous cells was maintained by Sijunzi decoction. T cell count was recovered and intestinal mucous barrier injury were lessened, and the bacterial positive rate in the internal organs was decreased . Conclusion Traditional Chinese herbal medicine Sijunzi decoction might be helpful in alleviation of postburn intestinal injury and in the prevention of intestinal bacterial translocation. [