裸鼠造血微环境损伤实验动物模型的建立

目的建立适合移植实验使用的裸鼠造血微环境损伤动物模型。方法分别用3．5Gy、5Gy、6．5Gy、8Gy4个^60Coγ辐射剂量照射裸鼠，检测经过辐照后各组裸鼠1、3、5、7、14、21d血象变化，检测辐射后1、7、14、21d裸鼠骨髓基质CFU—F形成情况，用流式细胞仪检测辐射后1、7、14、21d骨髓基质细胞VCAM-1的表达。结果8．0Gy辐射剂量裸鼠死亡率100%；3．5Gy、5Gy、6．5Gy辐射剂量组裸鼠的造血损伤均能在辐射后21天之内自我恢复；6．5Gy组骨髓CFU—F于移植后7d（22±6）、14d（33±6）较3．5Gy、5Gy组均具有显著降低（P〈0．01），VCAM-1表达于7d（21．67±7．45）、14d（22．33±9．07）、21d（23．33±4．51）较对照组显著降低（P〈0．01）、较3．5Gy组差异有显著性（P〈0．05），21d（23．33±4．51）较5Gy组（34．00±4．36）差异有显著性（P〈0．05）。结论6．5Gy是裸鼠造血微环境损伤实验动物模型的适宜辐射剂量。

Establishment of the hematopoietic inductive microenvironment damage model in nude mice

Objective To establish the hematopoietic inductive microenvironment （HIM） damage model for the transplant experiment in nude mice. Methods Irradiate the experimental nude mice by 3.5GY, 5GY, 6- 5GY and 8GY ^60Coγ respectively and detect the changes of hemogram at 1, 3, 5, 7, 14, and 21 days and the formation of CFU-F in marrow stroma at 1,7,14, and 21 days, and the expression of VCAM-1 at 1, 7, 14, and 21 days by flow cytometry. Results Irradiated with 8Gy, the death rate of nude mice was 100%. All the nude mice in 3.5Gy group, 5Gy group and 6.5Gy group could be recovered by themselves within 21 days after radiation. Moreover in the 6.5Gy group, the CFU- F was markedly lower at 7d（22±6） and 14d（33±6） than 3.5Gy and 5Gy groups（P〈0.01）. The expression of VCAM- 1 at 7d（21.67±7.45）, 14d（22.33±9.07）,and 21d（23.33±4. 51） was significantly lower than the control group （P〈 0.01）, and different from the 3.5Gy group（P〈0. 05）. The expression of VCAM-1 was significantly different between 5Gy group （34.00±4.36） and at 21d （23.33±4. 51 ,P〈0.05）. Conclusion 6. 5Gy is the suitable radiation dose for the HIM damage model in nude mice.