| 查尔酮对人胃癌BGC823细胞生物学行为影响的初步研究 |
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| 引用本文： | 王丽,阎蕾,张景怡,冯正明,贾光,张宝旭.查尔酮对人胃癌BGC823细胞生物学行为影响的初步研究[J].包头医学院学报,2004,20(4):265-267. |
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| 作者姓名： | 王丽 阎蕾 张景怡 冯正明 贾光 张宝旭 |
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| 作者单位： | 内蒙古科技大学公共卫生学院,内蒙古,包头,014010;北京大学医学部 |
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| 基金项目： | 国家"211"工程建设项目 |
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| 摘 要： | 目的:研究查尔酮对人胃癌BGC823细胞生长抑制、细胞周期阻滞及诱导细胞凋亡的作用,为寻找预防肿瘤药物提供一定的线索.方法:细胞生长抑制采用MTT比色法检测,细胞周期阻滞用流式细胞仪检测,细胞凋亡用流式细胞仪及琼脂糖凝胶电泳检测.结果:浓度为6.25、12.5、25和50μmol/L的查尔酮呈剂量依赖性抑制BGC823细胞增殖.查尔酮诱导细胞凋亡率是对照的2.71、2.79、4.74倍.琼脂糖凝胶电泳可观测到12.5μmol/L、25μmol/L有DNA"梯子"现象.结论:查尔酮可以抑制BGC823细胞生长,并呈剂量-反应关系.查尔酮有将BGC823细胞增殖周期阻滞在G0/G1期趋势,查尔酮可以抑制肿瘤细胞的生长和增殖.
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| 关 键 词： | 查尔酮 人胃癌BGC823细胞 细胞增殖抑制 细胞周期阻滞 细胞凋亡 |
| 文章编号： | 1006-740X(2004)04-0265-03 |
| 修稿时间： | 2004年10月8日 |
Elementary study of the Effect of Chalcone on Cellular Biological Behavior of Human Stomach Cancer BGC823 |
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| WANG Li,YAN Lei,ZHANG Jinyi,FENG Zhenming,JIA Guang,ZHANG Baoxu.Elementary study of the Effect of Chalcone on Cellular Biological Behavior of Human Stomach Cancer BGC823[J].Journal of Baotou Medical College,2004,20(4):265-267. |
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| Authors: | WANG Li YAN Lei ZHANG Jinyi FENG Zhenming JIA Guang ZHANG Baoxu |
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| Abstract: | Objective:To understand how chalcone inhibits the growth of human stomach cancer BGC823 ,arrests the cell cycle progress and induces cell apoptosis in order to explore a new clue to finding a cure for stomach cancer. Methods: Cytotoxicity was determinded by MTT chromatometry, cell cycle progress and apoptosis were determined by flow cytometry. DNA ladder was examined by Agarose Gel Electrophoresis. Results: Cytotoxicity induced by chalcone were increased in a dose - dependent manner ( r >0.91, P <0.01).6.25umol/L chalcone can arrest the cell cycle at the G0/G1 phase( P <0.05). Agarose Gel Electrophoresis showed that the phenomena of DNA "ladder" appeared in the chalcone group at the doses of 12. 5 ,25umol/L. Conclusion : chalcone can inhibit the growth of BGC823 and arrest the cell cycle. |
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| Keywords: | Chalcone BCC823 Apoptosis Cell cycle proress |
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