| 水飞蓟宾对培养心肌细胞感染CoxSackie B5病毒的保护作用 |
| |
| 引用本文： | 章同华 沈茜. 水飞蓟宾对培养心肌细胞感染CoxSackie B5病毒的保护作用[J]. 第二军医大学学报, 1990, 11(2): 143-146 |
| |
| 作者姓名： | 章同华 沈茜 |
| |
| 作者单位： | 长海医院心血管内科(章同华，王舒娅)，长海医院实验诊断科(沈茜) |
| |
| 摘 要： | 本文介绍用微孔培养板取代培养瓶培养新生大鼠心肌细胞,并用400 TCI_(50) 0.05 ml/孔CoxsackieB_5病毒感染心肌细胞.在感染后1,6、24及36h分别加入水飞蓟宾,观察到用水飞蓟宾后培养液中LDH、GOT均低于病毒对照组,感染后DNA合成率明显高于病毒对照组,以及96 h受感染细胞培养液中病毒滴度(Lg 3.95 TCID_(50))明显低于病毒对照组(Lg 6.95 TCID_(50)).本实验结果提示水飞蓟宾对Coxsackie B_5病毒感染培养的心肌细胞有明显保护作用,尤其是在感染后1～6 h给药,效果更好。
|
| 关 键 词： | 心肌细胞培养 病毒感染 水飞蓟宾 |
Effect of Silybin on Coxsackie B_5 Virus Infected New Born Rat Heart Cells in Culture |
| |
| Zhang Tonghua,et al. Effect of Silybin on Coxsackie B_5 Virus Infected New Born Rat Heart Cells in Culture[J]. Former Academic Journal of Second Military Medical University, 1990, 11(2): 143-146 |
| |
| Authors: | Zhang Tonghua et al |
| |
| Abstract: | The protective effect of silybin on the micro-model of Coxsackie B5 virus infected new born rat heart cell culture was observed. After inoculation with 400TCID Coxsackie B5 virus, the cardiac enzyme s-lactic acid dehydrogenase (LDH) and glutamic oxaloacctic transaminase (GOT) were much lower in the silybin-treated groups than in those groups of untreated, but the synthesis rate of DNA was higher. Virus titer in the supernatant of cultures was detected 96 h after virus challenge. The virus titer (Lg 3. 95 TCID50) of the silybin-treated was lower than in that of untreated groups (Lg 6. 95 TCID50). These results suggest that silybin may be a useful drug in treatment of acute viral myocarditis. |
| |
| Keywords: | cell culture Coxsackie virus silybin heart muscle |
| 本文献已被 CNKI 维普 等数据库收录！ |
