Development and Validation of an HPLC Method for Karanjin in Pongamia pinnata linn. Leaves |
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Authors: | S Katekhaye M S Kale K S Laddha |
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Institution: | 1Medicinal Natural Products Research Laboratory, Department of Pharmaceutical Science and Technology, Institute of Chemical Technology, Matunga, Mumbai-400 019, India |
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Abstract: | A rapid, simple and specific reversed-phase HPLC method has been developed for analysis of karanjin in Pongamia pinnata Linn. leaves. HPLC analysis was performed on a C18 column using an 85:13.5:1.5 (v/v) mixtures of methanol, water and acetic acid as isocratic mobile phase at a flow rate of 1 ml/min. UV detection was at 300 nm. The method was validated for accuracy, precision, linearity, specificity. Validation revealed the method is specific, accurate, precise, reliable and reproducible. Good linear correlation coefficients (r2>0.997) were obtained for calibration plots in the ranges tested. Limit of detection was 4.35 μg and limit of quantification was 16.56 μg. Intra and inter-day RSD of retention times and peak areas was less than 1.24% and recovery was between 95.05 and 101.05%. The established HPLC method is appropriate enabling efficient quantitative analysis of karanjin in Pongamia pinnata leaves. |
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Keywords: | HPLC karanjin Pongamia pinnata quantification validation |
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