血清HBV标志或HBsAg阴性慢性肝炎患者肝内HBV DNA原位杂交分析

本文采用原位杂交及免疫组化方法对23例血清HBV标志或HBsAg阴性慢性肝炎患者的HBV感染状态进行了研究。发现肝内乙肝病毒脱氧核糖核酸(HBV DNA),HBsAg,HBcAg的检出率分别为43.48％、38.89％.16.67％;肝内有HBV感染依据者占65.22％,表明半数以上的血清HBV标志或HBsAg阴性患者肝内仍然存在HBV复制或基因表达。含HBV DNA的肝细胞及表达HBsAg,HBcAg的肝细胞大多毗邻灶状坏死和(或)碎屑样坏死灶,提示HBV复制及其产物表达与患者的慢性化、活动性坏死性炎症密切相关。

Analysis of Intrahepatocellular HBVDNA in Serologic Markers or HBsAe Negative Patients with Chronic Hepatitis

To explore HBV infection status in a group of serologic HBV markers or HBsAg negative patients with chronic hepatitis, in situ hybridization to liver HBV DNA was carried out, in conjunction with detection of intrahepatic HBsAg and HBcAg. It was found that incidence of intrahepatocellular HBV DNA, HBsAg and HBcAg was 43.48% ,38.89% and 16.67% respectively, and 65.22% among the patients had HBV markers expression in the liver.These findings indicate that over half of patients with chronic hepatitis were still undergoing HBV infection, despite serologic HBV marker or HBsAg negative. Furthermore, we found that hepatocytes containing HBV DNA or surface or core antigen expression were often close to hepatic necrosis foci, suggesting that HBV replication and its antigen(s) expression in hepatocytes could be an explanation for chronic, active and necrotic inflammation occurring in the liver of the patients in question.