三七总甙对非酒精性脂肪肝大鼠肝组织细胞色素P450 2E1表达的影响

目的研究三七总甙对非酒精性脂肪肝大鼠肝组织细胞色素P450 2E1表达的影响。方法用脂肪乳剂灌胃法诱导大鼠非酒精性脂肪肝模型，并给予三七总甙干预，观察肝组织病理学形态的变化，采用免疫组化法检测肝组织细胞色素P450 2E1的表达，酶标仪法测定肝内丙二醛（MDA）、超氧化物岐化酶（SOD）、游离脂肪酸（FFA）含量。结果与模型组比较，三七总甙干预组肝细胞的脂肪变程度明显减轻，脂滴减少，CYP 2E1的表达受到明显抑制，肝内SOD活性升高，MDA、FFA显著降低。结论三七总甙能显著抑制大鼠脂肪肝CYP 2E1的表达，减轻脂质过氧化反应，因而具有防治脂肪肝的作用。

Effects of panax notoginsenosides on the expression of cytochrome P450 2E1 in liver tissues in rats with nonalcoholic fatty liver

Objective To explore the effects of panax notoginsenosides on the correlation between hepatocyte cytochrome P450 2E1 （CYP 2E1 ） expression and oxidation,antioxidation in rats with nonalcoholic fatty liver. Methods The SD rats were divided at random into normal control group,fatty liver model group induced by long-term gastric perfusion of lipid emulsion and panax notoginsenosides group. At the end of experiment,liver tissue was studied with histopathology and CYP2E1 antigen was detected with immunohistochemistry. Meawhile,the hepatic MDA,SOD and FFA contents were measured by biochemical analysis. Results Compared with model group,the fatty deposition decreased in livers in panax notoginsenosides group and the hepatocyte cytochrome P450 2E1 expression was inhibited. Meanwhile,the contents of MDA and FFA were significantly decreased,but SOD contents were significantly increased in liver. Conclusion Panax notoginsenosides can inhibit the hepatocyte cytochrome P450 2E1 expression in rats with fatty liver and reduce lipid peroxidation.