脂质过氧化对血管内皮细胞促增殖活性的影响

为探讨脂质过氧化对血管内皮细胞源生长因子的影响，以氢过氧化枯烯作用于培养的牛主动脉内皮细胞后，收集内皮细胞条件培养基，并用肝素亲和层析法处理，分组测定其对3T3细胞3H-胸腺嘧啶核苷掺入率的影响，并用放射免疫法测定各脂质过氧化作用组内皮细胞培养基中内皮素的含量。结果发现．氢过氧化枯烯使培养的内皮细胞的脂过氧化物蓄积增加，且内皮细胞条件培养基对3T3细胞的DNA合成有促进作用．对Swiss3T3细胞的促增殖活性主要存在于非肝素结合部分。氢过氧化枯烯作用后的内皮细胞的内皮素分泌水平比对照组增加。由此推测脂质过氧化作用引起的内皮细胞对平滑肌细胞的促增殖活性可能与内皮素等非肝素结合的分子有关。

Effect of Lipid Peroxidation of Vascular Endothelial Cells on Mitogenic Activities

Aim To investigate whether the lipid peroxidation of endothelial cells had any effect on the production of growth factors by them.Methods The bovine aortic endothelial cells were isolated and cultured using an enzyme-dispersal method, and the media conditioned by endothelial cells with lipid peroxidation induced by cumene hydroperoxide were collected. Then the endothelial cell-conditioned media (ECCM ) were treated by heparin-binding sepharose, and the mitogenic activity of ECCM for swiss 3T3 cells were determined by incorporation of 3H-thymidine into DNA of the cells. The concentration of endothelin in the media of endothelial cells were analysed by radioimmunoassay.Results Cumene hydroperoxide induced storage oflipid peroxide in cultured endothelial cells, and there was an obvious increase in the incorporation of 3H-thymidine into DNA in Swiss 3T3 cells treated with ECCM compared with the control. However, the mito-genic activity of ECCM for Swiss 3T3 cells were in non heparin-binding parts. Then the concentration of en-dothelin in the media of endothelial cells were treated by cumene hydroperoxide were increased obviously compared with the control.Conclusion It seems reasonable to believe that lipidperoxidation of endothelial cells induced by cumene hy-droperoxide leads to an increased synthesis and secre-tion of growth factors, and the mitogenic activities were due to non heparin-binding parts like as endothe-lin.