小鼠肝癌细胞(H22)源外泌体的体内抗肝癌作用研究

目的在荷瘤动物模型观察小鼠肝癌细胞（H22）来源的外泌体（exosomes）激发宿主抗肝癌免疫应答的效应。方法用自行分离纯化的小鼠肝癌细胞H22源外泌体免疫小鼠，然后给小鼠皮下注射H22肿瘤细胞，观察肿瘤的生长状况。用MTT法检测小鼠脾细胞增殖情况和细胞毒活性；用免疫组织化学法检测肿瘤组织中CD4^＋、CD8^＋T淋巴细胞浸润情况。结果该外泌体使肿瘤出现时间延迟，肿瘤生长缓慢，小鼠生存率提高。促进脾淋巴细胞增殖并增强其细胞毒活性，促进CD4^＋和CD^8＋T细胞活化。结论小鼠肝癌细胞（H22）源外泌体能诱导抗肿瘤应答，有效地诱导特异性的杀伤肿瘤细胞活性，有针对亲本细胞的免疫保护作用。

Antitumor effects of exosomes derived from a mouse hepatoma carcinoma cell line H22

Objective To investigate the induction of antitumor response and its immune mechanism of H22 cell-derived exosomes. Methods Fifteen BALB/c mice were immunized by H22 cell-derived exosomes three times at weekly intervals,then inoculated with the wild-type H22. Another 15 BALB/c mice that were injected with PBS were used as controls. In the 80-day observation period,the tumor growth and survival time were observed. MTT was used to determine lymphocyte proliferation and specific cytotoxicity of mice splenic ce...