| 细胞凋亡检测用于卵巢癌化疗药物敏感性试验及其与ATP法的比较 |
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| 引用本文： | 楼江燕,王和,刘珊玲,彭芝兰. 细胞凋亡检测用于卵巢癌化疗药物敏感性试验及其与ATP法的比较[J]. 四川大学学报(医学版), 2002, 33(2): 267-269 |
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| 作者姓名： | 楼江燕 王和 刘珊玲 彭芝兰 |
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| 作者单位： | 四川大学华西第二医院,妇产科,成都,610041;四川大学华西第二医院,妇产科,成都,610041;四川大学华西第二医院,妇产科,成都,610041;四川大学华西第二医院,妇产科,成都,610041 |
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| 基金项目： | 国家自然科学基金资助 (批准号 39470 72 1) |
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| 摘 要： | 目的 探讨细胞凋亡检测用于卵巢癌化疗药物敏感性试验的可行性。方法 采用末端标记法直接检测顺氯胺铂等 6种化疗药物诱导 4例卵巢癌实体瘤细胞凋亡的结果 ,并与三磷酸腺苷生物发光法 (ATP法 )检测结果进行对比。结果 同一种化疗药物对不同来源的癌细胞的细胞凋亡诱导程度不同 ,与 ATP法比较二者的检测结果存在一致性 (P0 =0 .75 )。结论 上述结果提示 ,细胞凋亡检测能直接用于了解药物诱导肿瘤细胞凋亡的程度 ,若作为一种药物敏感性试验方法与 ATP法组合应用 ,将有可能提高临床应用效果
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| 关 键 词： | 细胞凋亡 化学疗法 药物敏感性试验 三磷酸腺苷生物发光法 |
| 修稿时间： | 2001-11-20 |
Comparison Between Apoptosis Cells Count and ATP-Bioluminescence Assay in Ovarian Cancer Chemosensitivity Testing in vitro |
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| Lou Jiangyan,Wang He,Liu Shanling,Peng Zhilan. Comparison Between Apoptosis Cells Count and ATP-Bioluminescence Assay in Ovarian Cancer Chemosensitivity Testing in vitro[J]. Journal of Sichuan University. Medical science edition, 2002, 33(2): 267-269 |
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| Authors: | Lou Jiangyan Wang He Liu Shanling Peng Zhilan |
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| Affiliation: | Lou Jiangyan,Wang He,Liu Shanling,Peng Zhilan. Department of Obstetrics and Gynecology,West China Second Hospital,Sichuan University,Chengdu 610041,China |
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| Abstract: | Objective This study was aimed to evaluate the potentiality for the use of apoptosis cells count as a chemosensitivity testing in vitro for ovarian cancer. Methods An end-labelling assay was used to determine the apoptosis cells of 4 specimens of fresh ovarian cancer cells and the results were compared with that of ATP assay. Results It was found that chemotherapy can induce apoptosis,and the same chemotherapeutic agent can induce different apoptosis cells for different cancers. Conclusion The observed agreement between apoptosis cells count and ATP assay was 0.75. The above data demonstrate that apoptosis cells count can be used as a chemosensitivity testing for ovarian cancer,and a combination of these two methods may improve the clinical effects of chemotherapeutic agents. |
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| Keywords: | Apoptosis Chemotherapy Chemosensitivity testing ATP-bioluminescence assay |
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