Assignment of a structural gene forβ-glucuronidase to human chromosome C7

An electrophoretic technique was developed which allows the separation of human -glucuronidase (GUS EC 3.2.1.31) from the enzyme present in cultured murine, Chinese and Syrian hamster cells in one buffer system on Cellogel. Using this technique a number of independent human-mouse somatic cell hybrids have been analyzed for the segregation of GUS, other enzyme markers, and all human chromosomes. The results indicate that a structural gene for human -glucuronidase is located on chromosome C7.