南方红豆杉水提物诱导人肺腺癌A549细胞凋亡与紫杉醇抑瘤机制不同的研究

目的:初步探讨南方红豆杉水提物抗人肺腺癌A549细胞(以下简称"A549细胞")的作用机制,明确其与紫杉醇抗肿瘤机制的不同。方法:以流式细胞仪检测南方红豆杉水提物对A549细胞凋亡的影响,以紫杉醇为对照;以普通光学显微镜观察南方红豆杉水提物和紫杉醇对A549细胞形态的影响;以Western blotting检测细胞凋亡相关蛋白P-jnk的表达。结果:①流式细胞检测及光镜观察发现南方红豆杉水提物处理后A549细胞呈典型的早期凋亡表现(P<0.05,P<0.01),而紫杉醇组细胞以坏死及晚期凋亡表现为主(P<0.05,P<0.01),且其比例均呈现一定的剂量-效应关系;②Western blotting检测发现南方红豆杉水提物处理后A549细胞P38蛋白表达无明显变化、P-jnk蛋白随药物浓度增加表达减少(P<0.01)。结论:南方红豆杉水提物抗A549细胞的机制与紫杉醇不同,其作用机制主要与诱导肿瘤细胞凋亡有关。

Study on mechanisms of inducing apoptosis with aqueous extract of Taxus chinensis and paclitaxel inhibitory effect in human lung carcinoma A549 cells

Objective: To preliminary study the inhibitory mechanisms of aqueous extract of Taxus chinensis and differences with paclitaxel in vitro cultured human lung carcinoma A549 cells.Methods: Effects on cell apoptosis was inspected by flow cytometry;morphology of the A549 cell was observed by ordinary optical microscope;expression of apoptosis-related proteins P-jnk was detected by Western blotting.Results: ①Optical microscope and FCM found typical manifestations of early stage tumor cell apoptosis in aqueous extract of Taxus chinensis arm(P<0.05,P<0.01),meanwhile,the tumor cells showed manoifestations by necrosis and later stage apoptosis in paclitaxel arm(P<0.05,P<0.01).And a dose-effect curve were showed in all arms.②The results detected by Western blotting showed that P38 protein expression was unchanged,and P-jnk protein expression was decreased with the increase of drug concentration in aqueous extract of Taxus chinensis arms(P<0.01).Conclusion: The aqueous extract of Taxus chinensis has different anti-tumor mechanisms with paclitaxel,and these possibly related to inducing apoptosis of human lung carcinoma A549 cells.