犬小孢子菌微量液基稀释法抗真菌药敏试验的方法学研究

目的对北京儿童医院的头癣患者分离所得的182株犬小孢子菌进行抗真菌药敏试验条件的探索。方法对致病真菌进行取样、培养、鉴定、分离和纯化,经微量稀释法测定8种常见抗真菌药物的最小抑菌浓度,并对原药敏方案进行适当调整。结果受试菌株的接种浓度为1~3×104CFU/mL。除伊曲康唑、联苯苄唑、阿莫罗芬外,使用沙氏液体培养基测定的药物最小抑菌浓度均较使用RPM I-1640液体培养基时药物浓度明显升高。孵育温度为28℃,孵育时间为5 d。结论各种药物的最小抑菌浓度(m in im al inh ib itory concentration,M IC)之间的差异有统计学意义,M38-A方案有较好的重复性和稳定性,可作为一种较好的实验室方法被应用。

Research about the Broth Microdilution Antifungal Susceptibility Testing Condition for 182 Microsporum Canis

Objective Study on the broth microdilution antifungal susceptibility testing condition about 182 Microsporum canis derived from patients in Beijing Children's Hospital.Methods The vitro method was used for testing filamentous fungi.We approached the broth microdilution antifungal susceptibility testing condition for Microsporum canis and tested the minimal inhibitory concentration(MIC) of 8 common antifungal drugs.Results The broth microdilution antifungal susceptibility testing condition for Microsporum canis: inoculum size ranged from 1×10⁴ to 3×10⁴ CFU/mL;MICs obtained from different medium at the same incubation time for the same isolate were significantly different,MICs were consistently higher with SDB than with RPMI-1640,when itraconazole,bifonazole,amorolfine were tested,no parameter had any influence on MICs;incubation temperature was 28 ℃,Microsporum canis produced clearly detectable growth after 5 d.Conclusion With good reproducibility and feasibility,the M38A procedure can be used for the antifungal susceptibility test to dermaphytes in the laboratories.