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| 疟原虫雌性配子体特异性表面蛋白Pys47真核表达及免疫效应评价 | |
| 引用本文： | 郑丽,冯辉,潘艳艳,李莹,孟红蕊,周泓旭,曹雅明. 疟原虫雌性配子体特异性表面蛋白Pys47真核表达及免疫效应评价[J]. 寄生虫与医学昆虫学报, 2011, 18(3): 135-138. DOI: 10.3969/j.issn.1005-0507.2011.03.002 |
| 作者姓名： | 郑丽 冯辉 潘艳艳 李莹 孟红蕊 周泓旭 曹雅明 |
| 作者单位： | 中国医科大学基础医学院免疫学教研室,沈阳,110001 |
| 摘 要： | P47是特异性表达于疟原虫雌性配子体以及雌性配子表面的蛋白,为了评价其作为传播阻断疫苗候选抗原的免疫效果及其效应,本研究将约氏疟原虫Pys47完整编码区基因序列克隆入真核表达载体pcDNA3.1+,获得核酸质粒,肌肉注射BALB/c小鼠.通过ELISA方法检测免疫小鼠血清滴度,IFA检测疫苗的免疫活性以及免疫血清对有性... |
| 关 键 词： | 约氏疟原虫 性别-特异性蛋白 Pys47 传播阻断效应 |
Eukaryotic Expression and Immunological Effect of Specific Surface Protein Pys47 of Plasmodium yoelii Female Gametocyte |
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| ZHENG Li,FENG Hui,PAN Yan-Yan,Li Ying,MENG Hong-Rui,ZHOU Hong-Xu,CAO Ya-Ming. Eukaryotic Expression and Immunological Effect of Specific Surface Protein Pys47 of Plasmodium yoelii Female Gametocyte[J]. Acta Parasitologica et Medica Entomologica Sinica, 2011, 18(3): 135-138. DOI: 10.3969/j.issn.1005-0507.2011.03.002 | |
| Authors: | ZHENG Li FENG Hui PAN Yan-Yan Li Ying MENG Hong-Rui ZHOU Hong-Xu CAO Ya-Ming |
| Affiliation: | ＊ ( Department of Immunology, China Medical University, Shenyang 110001, China) |
| Abstract: | P47 is a protein expressed only in female gametocyte and located on the surface of female gametes of malaria parasites. In present study, the complete sequence of Plasmodlum yoelii Pys47 was amplified by PCR and cloned into eukaryotic expression vector pcDNA3.1 ＋. Gene sequencing and restriction enzyme digestion confirmed the successful construction of Pys47 plasmid. The transmission blocking effect of immune sera from BALB/c mice vaccinated with the DNA plasmid Pys47 was determined by IFA, but the anti-sera couldn＇t markedly inhibit the development of the protozoa from gametocyte into zygotes and ookinetes when the parasites in sexual stage were cultured with anti-sera in vitro. The results of present study implied that the specific surface protein Pys47 may not be an ideal candidate of immunizing antigen for transmission-blocking vaccine. |
| Keywords: | Plasmodium yoelii Sex-specific protein Pys47 Transmission blocking effect |
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