灵芝抗癌活性及放化疗对HL-7702细胞的保护作用

目的：研究灵芝精粉（灵芝醇提物+水提物）、灵芝精粉氯仿提取物、氯仿提取后的醋酸乙酯提取物、2次提取后的剩余物对肝癌BEL-7402和胃癌MGC-803细胞的抑制效果及其对HL-7702细胞顺铂损伤、⁶⁰Coγ射线辐射损伤的修复与保护作用。方法: 采用MTT法检测灵芝抗癌活性及放化疗时对HL-7702细胞保护作用。结果: 灵芝氯仿提取物的抗癌活性为最强：在0.125 mg·mL^-1时,对癌细胞的抑制率已超过50％。对已受顺铂损伤的HL-7702细胞,4种提取物未能显示出修复作用,但氯仿提取物预处理可显著降低其损伤程度。对受辐射的HL-7702细胞,仅氯仿提取物对中、高剂量照射下的辐射损伤体现一定的修复作用；但4种提取物预处理均可降低其辐射损伤的程度。结论: 灵芝氯仿提取物对癌细胞的抑制作用,对正常细胞放化疗损伤的保护作用,均体现了显著的效果。

Antitumor activity of extracts of Ganoderma lucidum and their protective effects on damaged HL-7702 cells induced by radiotherapy and chemotherapy

OBJECTIVE: To study the inhibitory effect of Ganoderma lucidum, the extract of chloroform, the extract of ethyl acetate and the remains after two-time extraction on BEL-7402 and MGC-803 cells and their protective effects on HL-7702 cells pre-and post-exposed to cisplatin (DDP) and various doses of 60Co gamma irradiation. METHOD: The antitumor activity and protective effects on damaged HL-7702 cells induced by radiotherapy and chemotherapy of ganoderma lucidum were determined by MTT technique. RESULT: The anticancer activity of the extract of chloroform Ganoderma lucidum was the best: at the concentration of 0.125 mg x mL(-1), the inhibitory rate was over 50%. To the HL-7702 cells damaged by DDP, four kinds of extracts didn't exert restoring effect, but the pretreatment with the extract of chloroform reduced the damaged degree significantly. To the 60Co gamma irradiated HL-7702 cells, only the extract of chloroform exerted restoring effect to some extent when exposed to middle or high dose of irradiation. The pre-administration of four kinds of extracts reduced the damaged degree by radiation. CONCLUSION: The extract of chloroform exerts notable antitumor effects on cancer cells and protective effects on damaged normal cells induced by radiotherapy and chemotherapy.