| 半枝莲醇提工艺的优化及体外抗氧化活性评价的研究 |
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| 引用本文： | 薛海萍,朱春赟,芮欣忆,张婷.半枝莲醇提工艺的优化及体外抗氧化活性评价的研究[J].中国实验方剂学杂志,2010,16(14):12-16. |
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| 作者姓名： | 薛海萍 朱春赟 芮欣忆 张婷 |
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| 作者单位： | 上海中医药大学,上海,201203 |
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| 基金项目： | 上海市教育委员会科研创新项目(自然科学类)一半枝莲总黄酮提取物的制备工艺及质量标准研究 |
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| 摘 要： | 目的:用正交试验法对半枝莲的乙醇提取工艺进行优化研究,通过体外试验对提取物的抗氧化活性进行评价。方法:以浸膏得率、总黄酮和野黄芩苷从生药中的提取率为评价指标,选择乙醇体积分数(%)、乙醇用量(倍)、提取时间(h)和提取次数(次)为考察因素,利用正交试验L9(34)确定半枝莲的最佳醇提工艺。基于氧化还原反应原理的FRAP法用于总抗氧化能力的评估,DPPH法用于评价自由基清除能力。结果:最佳工艺条件为用药材10倍量的75%乙醇加热回流提取3次,每次1 h。半枝莲提取物以浓度依赖关系发挥着很好的抗氧化作用,对DPPH自由基具有较强的清除和抑制作用。结论:该提取工艺具有较佳的重复性和可行性。半枝莲醇提物显示了较好的抗氧化活性,值得进一步开发研究。
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| 关 键 词： | 半枝莲 正交试验 总黄酮 野黄芩苷 抗氧化 |
| 收稿时间： | 2010/6/17 0:00:00 |
Studies on Optimum Ethanol Extraction Process and Antioxidant Activities in vitro of Scutellaria barbata |
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| XUE Hai-ping,ZHU Chun-yun,RUI Xin-yi and ZHANG Ting.Studies on Optimum Ethanol Extraction Process and Antioxidant Activities in vitro of Scutellaria barbata[J].China Journal of Experimental Traditional Medical Formulae,2010,16(14):12-16. |
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| Authors: | XUE Hai-ping ZHU Chun-yun RUI Xin-yi and ZHANG Ting |
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| Institution: | XUE Hai-ping,ZHU Chun-yun,RUI Xin-yi,ZHANG Ting(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China) |
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| Abstract: | Objective:To study the optimum ethanol extraction process and antioxidant activities in vitro of Scutellaria barbata D.Don.Method:The optimum ethanol extraction process was established with the orthogonal design.The extraction rate of extractum,total flavonoids and scutellarin from crude material were chosen as the as-sessment guideline.Antioxidant activities in vitro of the extract were evaluated by FRAP assay and DPPH assay compared with Trolox.Result:The optimum ethanol extraction process conditions were... |
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| Keywords: | FRAP DPPH |
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