建立阿尔茨海默症的转基因动物模型

目的 建立阿尔茨海默症转基因动物模型，为病因研究和药物筛选提供了一个合适的运动模型。方法 通过显微注射方法将外源ＤＮＡ注射到小鼠受精卵的雄性原核，移植到假孕母鼠的输卵管。仔鼠出生后，经ＰＣＲ及ＰＣＲ－Ｓｏｕｔｈｅｒｎ杂交检测阳性小鼠。结果共注射８８７枚受精卵，存活４８２枚，称卵后产仔３５只，阳性３只，阳性鼠分别传代，在Ｆ２代获得纯合子后建系。免疫组织化学显示在大脑皮层、小脑及海马的神经细胞有ＡＰ

ESTABLISHMENT OF THE TRANSGENIC MODEL OF ALZHEIMER DISEASE

Objective\ To establish a transgenic model of Alzheimer disease. Methods\ We constructed the transgene containing the human APP cDNA 695 V717I driven by PDGF promoter which expresses specifically in nervous system. The transgene was microinjected into the male pronucleus of the zygotes. The tail DNA of pups was tested by PCR and Southern blot. Results\ 887 embryos were microinjected, 482 were implanted to 20 recipient pseudopregnant mice, 3 of 35 pups carrying the transgene. Each Heterozygous founders generated a transgenic line that was homozygous for the transgene. Immunocytochemistry revealed plaques in pallium, cerebellum, and hippocampus. Conclusions\ Resembling animal model of the human pathogenic mechanism for the screening of proper remedies established in our laboratory and could be used for further studied.