注射用消障素结膜下注射对豚鼠d-半乳糖性白内障的防治作用

目的 :探讨注射用消障素对豚鼠实验性d 半乳糖性白内障的防治作用。方法 :用 0 4 %d 半乳糖 0 2mL 眼给豚鼠球后注射 ,连续 19d ,造成d 半乳糖性白内障模型。注射用消障素治疗起始于诱发模型当日 ,高、低剂量实验组分别结膜下注射0 0 4mL(原液及 1∶1稀释液 ) 眼 ,隔日 1次 ,共 10次。于实验结束之日进行裂隙灯显微镜摄影 ,对各组实验动物晶状体混浊度进行分析比较 ,并测定其MDA含量及SOD活性。结果 :注射用消障素高、低 2个剂量组均能显著抑制豚鼠d 半乳糖性白内障晶状体混浊度的进展 ,作用与阳性对照药白内停组相仿 ;并可显著降低其晶状体MDA的含量 ;对SOD活性则无明显影响。结论 :注射用消障素结膜下注射 ,可明显抑制豚鼠d 半乳糖性白内障的发生发展 ,并对其晶状体脂质过氧化损伤具有一定的保护作用。

The Preventive and Therapeutic Effects of Subconjunctival Injection of Xiaozhangsu on Experimental D-Galactose Catarac of Guinea Pig

To study on the preventive and therapeutic effect of Xiaozhangsu Injection (XZS) on cataract induced by D galactose in guinea pigs, the D galactose cataract model was made by means of retrobulbar injection of 0.4% D galactose at the dose of 0.2ml/day in each eye to guinea pigs for continuous 19 days. The subconjunctival injection of 0.04 ml XZS with high dose and low dose was used at the same day of model induction and was done once every other day for 10 times. The lenses were photographed on the day or experimental end. The opacities of lenses were compared and analyzed. Simultaneously, the contents of MDA and the activities of SOD in lenses were assayed. The results showed XZS was able to inhibit the development of opacity and decreased significantly the content of MDA. But it has no obvious effect on the activity of SOD.