葡萄籽提取物原花青素对小鼠负重游泳时间的影响

背景原花青素是一种葡萄籽提取物,具抗氧化清除自由基的作用.研究发现运动引起骨骼肌自由基和其他它形式的活性氧增加是导致骨骼肌损伤和疲劳的重要原因之一.补充抗氧化剂能阻抑这种改变,提高运动能力.目的研究葡萄籽提取物原花青素的抗疲劳作用. 设计以实验动物为研究对象,完全随机设计,对照实验研究.单位省级疾病预防控制中心毒理室. 材料实验于 2001- 09/12在江苏省疾病预防控制中心毒理室完成.采用雄性昆明种小鼠,普通级,购自中国药科大学实验动物中心.动物合格证号SCXK(苏 ) 2002-0011;实验动物环境设施合格证号 SYXK(苏) 2001-0004号.按体质量随机分为溶剂对照组和3个实验组,即原花青素低、中、高剂量组,每组 10只,共 120只.实验组每天经口给予原花青素.方法3个实验组分别经口给与原花青素 1.7, 16.7, 50 mg/kg体质量,对照组给与双蒸水,连续30 d.给与原花青素 30 d后测定小鼠负重游泳时间,肝糖元含量,血乳酸及血红蛋白含量.主要观察指标 小鼠负重游泳时间,肝糖元含量,血乳酸及血红蛋白含量.结果各剂量组小鼠负重游泳时间均比对照组的延长,中、高剂量组分别达到(17.84± 8.48) min和 (25.80± 7.45) min,差异具极显著意义 (P< 0.01),各剂量组小鼠运动后血乳酸含量均低于对照组,高剂量组(6.78± 2.45) mmol/L与对照组 (9.98± 1.22) mmol/L)相比差异具极显著意义(P< 0.01);肝糖元含量均高于对照组, 中剂量组 [(1 244.65 ± 177.58)mg/100 g肝 ],高剂量组 [(1 383.96± 141.20) mg/100 g 肝 ]与对照组 [(817.67± 114.72)mg/100 g 肝 ]相比差异分别具显著和非常显著性意义 (P< 0.05,P< 0.01). 结论 原花青素具有抗疲劳作用.

Effect of grape seed extract proanthocyanidin on loaded swimming time in mice

BACKGROUND:Proanthocyanidin,a kind of grape seed extract(GSE),has the effect of anti-oxidation and elimination of free radicals.Research finds that increase of free radicals in skeletal muscle and active oxygen in other forms induced by exercise is one of the important causes of the injury and fatigue of skeletal muscle. Supplement of antioxidant can inhibit this kind of change and improve motor ability. OBJECTIVE:To study the anti-fatigue effects of proanthocyanidin. DESIGN:A completely randomized controlled experimental study taking experimental animals as the subjects. SETTING:A toxicological department of a provincial center for disease control and prevention. PARTICIPANTS:The experiment was completed in the Department of Toxicology,Jiangsu Center for Disease Control and Prevention from September to December 2001.Male mice of Kunming strain and common grade[certification number:SCXK(Su) 2002-0011;certification number of environmental equipment for experimental animal:SYXK(Su) 2001-0004]were purchased from the Experiment Animal Center of Chinese Pharmacological University.The 120 mice were randomly divided according to body mass into control group and 3 experiment groups, namely,low-,medium-and high-dose GSE groups,with 10 mice in each group.The mice in the 3 experiment groups received oral administration of GSE every day. INTERVENTIONS:The mice in the 3 experiment groups received oral administration of GSE of 1.7, 16.7 and 50.0 mg/kg body mass,respectively,while those in control group were only given twice distilled water for 30 consecutive days. After GSE treatment for 30 days,the loaded swimming time, contents of hepatic glycogen,blood lactic acid(LA) and hemoglobin were measured. MAIN OUTCOME MEASURES:Loaded swimming time, hepatic glycogen,LA and hemoglobin contents. RESULTS:The loaded swimming time of mice in experiment groups was significantly prolonged as compared with that in control group (P< 0.01),with(17.84± 8.48) and(25.80± 7.45) minutes in medium-and high-dose GSE groups,respectively.LA content after exercise in experiment groups was all lower than that in control group,with greatly significant difference between high-dose GSE group[(6.78± 2.45) mmol/L] and control group[(9.98± 1.22) mmol/L](P< 0.01).The content of hepatic glycogen after exercise in experiment groups was higher than that in control group; medium-dose GSE group[(1 244.65(177.58) mg/100 g liver mass]and high-dose GSE group[(1 383.96± 141.20) mg/100 g liver mass]differed greatly significantly from control group[(817.67(114.72) mg/100 g liver mass](P< 0.05,P< 0.01). CONCLUSION:Grape seed extract proanthocyanidin has an anti-fatigue effect.