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| Isolation and Characterization of 44.6 kDa Protein from Schistosoma japonicum Male Worm | |
| 引用本文： | 陈欲晓,易新元,曾宪芳,袁仕善,张顺科,LarryMCREYNOLDS. Isolation and Characterization of 44.6 kDa Protein from Schistosoma japonicum Male Worm[J]. 中华微生物学和免疫学杂志(英文版), 2004, 2(2): 136-139 |
| 作者姓名： | 陈欲晓 易新元 曾宪芳 袁仕善 张顺科 LarryMCREYNOLDS |
| 作者单位： | Department of Immunology，Xiangya School of Medicine，Central South University，Changsha 410078，China，Department of Parasitology，Xiangya School of Medicine，Central South University，Changsha 410078，China，Department of Parasitology，Xiangya School of Medicine，Central South University，Changsha 410078，China，Department of Parasitology，Xiangya School of Medicine，Central South University，Changsha 410078，China，Department of Parasitology，Xiangya School of Medicine，Central South University，Changsha 410078，China，Molecular Parasitology Group New England Biolabs Inc.，Beverly，MA0191，LMcreynolds，USA |
| 基金项目： | This work was supported by WHO TDR (No.980268) |
| 摘 要： | Schistosomes are hermaphrodite. The male worm has somespecific antigen different from the female′s [1,2]. Explo ration and characterization of the male worm antigen com ponents may be of great importance to investigation andprevention of schistosomiasis… |
| 关 键 词： | 44.6kDa蛋白质 免疫机制 疫苗接种 血吸虫 |
Isolation and Characterization of 44.6 kDa Protein from Schistosoma japonicum Male Worm |
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| Yuxiao CHEN,Xinyuan YI,Xianfang ZENG,Shishan YUAN,Shunke ZHANG and Larry MCREYNOLDS. Isolation and Characterization of 44.6 kDa Protein from Schistosoma japonicum Male Worm[J]. Chinese Journal of Microbiology and Immunology, 2004, 2(2): 136-139 | |
| Authors: | Yuxiao CHEN Xinyuan YI Xianfang ZENG Shishan YUAN Shunke ZHANG Larry MCREYNOLDS |
| Affiliation: | [1]DepartmentofImmunology,XiangyaSchoolofMedicine,CentralSouthUniversity,changsha410078,China [2]DepartmentofParasitology,XiangyaSchoolofMedicine,CentralSouthUniversity,Changsha410078,China |
| Abstract: | Soluble male worm antigen of Schistosoma japonicum ( Sj ) was investigated for development of new vaccine candidate. SDS-PAGE and Western blotting were performed to compare the difference between soluble antigens from worms of different sex. Mice vaccination with the testing purified protein was followed by Sj cercariae challenge to detect the protective effect against Sj . Sixteen bands were seen for the soluble male worm antigen and 12 for the female worm. In addition, a distinct band of 44.6 kDa from the male worm antigen was observed, and its antigenicity was demonstrated by Western blotting. This 44.6 kDa protein could induce significant worm and egg reduction rate in mice (39.31%, 41.98%, P <0.001). In this study a 44.6 kDa protein was isolated and partially characterized. Its antigenicity, immunogenicity and the partial immune protection suggest its potential vaccine candidte against Sj . |
| Keywords: | Schistosoma japonicum Male adult worm Antigenicity |
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