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| 内皮素1对人牙髓细胞胶原合成的影响 | |
| 引用本文： | 严焱,刘正. 内皮素1对人牙髓细胞胶原合成的影响[J]. 中华口腔医学杂志, 1999, 34(3): 162-164 |
| 作者姓名： | 严焱 刘正 |
| 作者单位： | 上海第二医科大学口腔医学院 |
| 摘 要： | 目的 探讨内皮素１地人牙髓细胞胶原合成的影响。方法 采用细胞培养、酶联免疫吸附分析及免疫组化染色等方法，就ＥＴ－１对ＨＤＰＣ胶原合成的影响进行了研究。结果 ＥＴ－１较对照组除２４小时时相外，均有不同程度的促ＨＤＰＣ可溶性Ｉ型胶原合成作用，但对Ⅲ型胶原作用不明显；经免疫组化染色证实；ＥＴ－１尚可同时促进细胞非溶解性Ｉ型胶原合成。结论 ＥＴ－１促进ＨＤＰＣＩ型胶原合成增加，可能对牙髓组织的自身修复起作 |
| 关 键 词： | 内皮素 胶原合成 免疫组织化学 牙髓细胞 |
Effects of endothelin 1 on the collagen synthesis of cultured human dental pulp cells |
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| YAN Yan,LIU Zheng. School of Stomatology,Shanghai Second Medical University,Shanghai. Effects of endothelin 1 on the collagen synthesis of cultured human dental pulp cells[J]. Chinese journal of stomatology, 1999, 34(3): 162-164 | |
| Authors: | YAN Yan LIU Zheng. School of Stomatology Shanghai Second Medical University Shanghai |
| Affiliation: | School of Stomatology, Shanghai Second Medical University, Shanghai 200011. |
| Abstract: | Objective To study the effects of endothelin1(ET1) on the collagen synthesis of cultured human dental pulp cells. MethodsCell cultured, enzyme linked immmun sorbent assay (ELISA),and ABC immunhistochemical staining were used in the study. Results ET1 increased the synthesis of soluble type I collagen in different periods besides the 24h group, and had no effects on the type III collagen; immunohistochemical staining revealed that ET1 also increased the synthesis of unsoluble type I collagen. Conclusion ET1 can stimulate type I collagenic synthesis of human dental pulp cells and might have an effect on pulp selfrestoration. |
| Keywords: | ndothelins Collagen Enzymelinked immmunosorbent assay Immunohistochemistry J |
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