电针夹脊穴对关节炎大鼠的治疗作用及对T细胞亚群的影响

目的:从细胞免疫的角度探讨电针夹脊穴对佐剂性关节炎(AA)大鼠的疗效机制。方法:以随机数字表法将30只雄性Wistar大鼠分为3组,每组10只。①正常组:不造模,不电针。②模型组:大鼠右后足掌皮下注射弗氏完全佐剂0.1 mL,造成佐剂性关节炎模型。正常组与模型组每日以与电针组相同的方式捆绑固定30 min,连续7 d。③电针组:于造模当天进行电针L3、L5夹脊穴30 min,每日1次,连续治疗7 d。检测其痛阈、肿胀度及T细胞亚群的变化。结果:电针夹脊穴明显提高AA大鼠痛阈,显著抑制致炎大鼠原发足肿胀,显著提高CD8+细胞百分率,降低CD4+/CD8+比值。结论:电针夹脊穴有明显的抗炎镇痛作用,其机制可能与调整AA大鼠紊乱的T细胞亚群有关。

Effect of Electroacupuncture of Jiaji (EX-B 2) on Pain, Paw-swelling and T-lymphocyte Subsets in Adjuvant Arthritis Rats

Objective: To observe the effect of electroacupuncture(EA) on pain threshold,paw swelling and serum T-lymphocyte subsets in adjuvant-induced arthritis(AA) rats so as to study the mechanism of EA in cellular immunity.Methods: Thirty male Wistar rats were divided into control,model and EA groups using random number table method,with 10 rats in each group.AA model was established by injection of complete Freund adjuvant(0.1%,(0.1 mL)) into the subcutaneous tissue of the right hindpaw.EA((4/60 Hz),dense-sparse pulses,(1 mA)) was applied to bilateral Jiaji(EX-B 2) points of L_3 and L_5 for 30 minutes,once daily,continuously for 7 days.The pain threshold(latency of paw withdrawal induced by radiant heat irradiation),paw swelling rate [(paw volume of post-injection of CFA-that of pre-injection)/that of pre-injection ×100%] and serum T-lymphocyte subsets(CD_4~+ and CD_8~+) were detected respectively.Results: Compared with normal control group,the paw volume(PV) and paw swelling rates of model group and EA group increased significantly while the paw withdrawal latencies on the focus side shortened markedly one day after injection of CFA(P<0.01).On the 7~(th) day,PV and paw swelling rate of model group were still significantly higher than those of control group(P<0.01);while in comparison with model group,both PV and paw swelling rate decreased considerably(P<0.05) and the paw withdrawal latency increased markedly in EA group(P<0.05).It indicated that EA could effectively relieve pain and swelling of the focal hindpaw.In comparison with control group,the percentages of CD_4~+ and CD_8~+ of model group decreased apparently(P<0.05),and CD_4~+/CD_8~+ increased significantly(P<0.05).Comparison between model and EA groups showed that the percentage of CD_8~+ increased evidently(P<0.01) and the ratio of CD_4~+ to CD_8~+ decreased significantly in EA group(P<0.05).Conclusion: EA of Jiaji(EX-B 2) has anti-inflammatory and analgesic effects,which maybe related to EA induced favorable regulation on T-lymphocyte subsets in AA rats.