VEGF-C与PDGF-BB在结肠癌组织中的表达

目的探讨结肠癌组织中血小板衍生生长因子BB（PDGF-BB）、血管内皮生长因子C（VEGF-C）的表达,及其与肿瘤临床病理参数之间的关系;分析在结肠原发癌与相应淋巴结转移癌中VEGF-C、PDGF-BB表达是否存在差异。方法收集结肠癌组织50例、淋巴结转移癌30例及癌旁正常组织15例,应用免疫组化SP法检测VEGF-C,PDGF-BB在癌旁正常组织、结肠癌和淋巴结转移癌中的表达。结果结肠癌组织中VEGF-C阳性表达率显著高于癌旁正常组织（P〈0.05）;VEGF-C的表达与结肠癌的浸润深度、淋巴结转移有关（均P〈0.05）,与病人性别、肿瘤大小及分化程度无关（P〉0.05）。PDGF-BB在结肠癌组织中的阳性表达率显著高于癌旁正常组织（P〈0.05）;PDGF-BB的表达与肿瘤的浸润深度有关（P〈0.05）,与病人的性别、肿瘤的大小、分化程度及淋巴结转移无关（P〉0.05）。PDGF-BB、VEGF-C2者之间的表达呈显著正相关（r=0.572,P〈0.05）。PDGF-BB、VEGF-C在淋巴结转移癌中的表达率均高于其相应的原发癌组织,但无统计学意义（均P〉0.05）。结论VEGF-C表达与结肠癌的浸润及淋巴结转移有关;PDGF-BB的表达与结肠癌浸润有关。PDGF-BB、VEGF-C在结肠癌中的表达呈正相关。肿瘤原发癌与淋巴结转移癌中VEGF-C、PDGF-BB的表达无异质性。

Expression of PDGF-BB and VEGF-C in Human Colonic Carcinoma

Objective To investigate the relationship between the expression of platelet derived growth factor BB （PDGF-BB）,vascular endothelial growth factor C（VEGF-C）and the clinicopathologic parameters in colonic carcinoma,and to explore the difference of VEGF-C and PDGF- BB expression in primary tumor and metastatic lymph node. Methods The expression of PDGF- BB and VEGF-C was detected by immunohistochemieal method in 50 colonic carcinomas,30 metastatic lymph nodes and 15 pericarcinoma normal tissues. Results The positive rate of VEGF-C was significantly higher in colonic carcinoma than in pericarcinoma normal tissue（P〈0.05）. The expression of VEGF-C was significantly correlated with the depth of invasion and lymph node metastasis（P〈0.05） ,but was not correlated with the gender, tumor size and differentiation degree （P〉0.05）. The positive rate of PDGF-BB was also higher in colonic carcinoma than in pericarcinoma normal tissue（P〈0.05）. The expression of PDGF-BB was significantly correlated with the depth of invasion（P〈0.05）, but was not correlated with the gender, tumor size,differentiation degree and lymph node metastasis（P〉0.05）. There was a significant positive correlation between the expression of PDGF-BB and VEGF-C（r= 0. 572, P〈0.05）. The positive rate of PDGF-BB and VEGF-C were higher in metastatic lymph node than in the primary tumor, but there was no statistical significance（P〉0.05）. Conclusion VEGF-C expression have a relationship with the depth of invasion and lymph node metastasis of colon caicinoma. PDGF-BB expression is correlated with the depth of invasion of primary tumor. The expression of PDGF-BB and VEGF-C have a positive correlation. There has no difference of PDGF-BB and VEGF-C expression in primary tumor and metastatic lymph node.