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| 死亡相关蛋白小分子干扰RNA对帕金森病大鼠黑质酪氨酸羟化酶表达的影响 | |
| 引用本文： | 亓爱芹,薛莉,谢安木. 死亡相关蛋白小分子干扰RNA对帕金森病大鼠黑质酪氨酸羟化酶表达的影响[J]. 中国临床神经科学, 2011, 19(2): 150-154 |
| 作者姓名： | 亓爱芹 薛莉 谢安木 |
| 作者单位： | 1. 青岛大学医学院附属医院东区神经内科,266003 2. 青岛大学医学院附属医院康复科,266003 |
| 摘 要： | 目的:利用动物模型探讨死亡相关蛋白(FADD)小分子干扰RNA(siRNA)对帕金森病(PD)大鼠黑质酪氨酸羟化酶(TH)表达的影响。方法:40只雄性Wistar大鼠随机分为4组。经立体定向定位黑质,PD组:注入6-羟基多巴(6-OHDA);siRNA组:注入FADD siRNA2次后再注入6-OHDA;SiRNA阴性对照组:注入FADD siRNA阴性对照物2次后再注入6-OHDA;正常对照组:注入抗坏血酸生理盐水。各组大鼠术后4周处死,取鼠的术侧中脑黑质,利用原位杂交检测FADD、TH mRNA表达。结果:PD组与正常对照组比较FADD mRNA表达显著增加(P<0.01);siRNA组与PD组比较FADD mRNA表达显著减少(P<0.05);siRNA阴性对照组与正常对照组比较FADD mRNA表达量无显著差异(P>0.05)。PD组与正常对照组比较TH mRNA表达显著减少(P<0.01);siRNA组与PD组比较TH mRNA表达显著增加(P<0.05);siRNA阴性对照组与正常对照组比较TH mRNA表达量差异无统计学意义(P>0.05)。结论:FADDsiRNA可能通过抑制凋亡对PD大鼠模型有一定的保护和治疗作用。 |
| 关 键 词： | 死亡相关蛋白小分子干扰RNA 酪氨酸羟化酶 帕金森病 大鼠 |
Effect of FADD siRNA on Tyrosine Hydroxylase in Parkinson's Disease Model Rats |
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| QI Ai-Qin,XUE Li,XIE An-Mu. Effect of FADD siRNA on Tyrosine Hydroxylase in Parkinson's Disease Model Rats[J]. Chinese Journal of Clinical Neurosciences, 2011, 19(2): 150-154 | |
| Authors: | QI Ai-Qin XUE Li XIE An-Mu |
| Affiliation: | QI Ai-Qin,XUE Li~2,XIE An-Mu~1 1 Department of Neurology,2 Department of Rehabilitation,the Affiliated Hospital of Medical College,Qingdao University,Qingdao 266003,China |
| Abstract: | Aim:To explore the effect of Fas-associated death domain(FADD) siRNA on tyrosine hydroxylase (TH) on Parkinson's disease modal.Methods:A total of 40 male Wistar rats were randomly assigned to four groups,10 rats in each group.Ten rats in PD group were prepared to be hemiparkinsonian models induced by unilateral stereotaxic infusion of 6-hydroxydopamine(6-OHDA) in right substantia nigra of midbrain.FADD siRNA sequences in FADD siRNA group were separately injected into the right substantia nigra of siRNA,then... |
| Keywords: | Fas-associated death domain(FADD) siRNA tyrosine hydroxylase Parkinson's disease rat |
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