抗纤软肝方对刀豆蛋白A致小鼠免疫性肝损伤的保护作用

目的探讨抗纤软肝方对刀豆蛋白A（ConA）所致免疫性肝损伤的保护作用。方法72只NIH小鼠随机分为6组,分别为正常对照组,模型组、抗纤软肝方高剂量组、中剂量组、低剂量组（20.0、10.0、5.0g/kg）和联苯双酯（150mg/Kg）治疗组。除正常对照组外,其他组小鼠于实验首日静脉注射ConA20mg/Kg,抗纤软肝方高、中、低剂量组和联苯双酯组均灌胃给药,每天1次,连续3d,末次给药后4h,再次静脉注射ConA20mg/kg,8h后检测血浆谷丙转氨酶（ALT）活性、干扰素γ（IFN-γ）和肿瘤坏死因子（TNF-α）含量,观察肝组织病理学变化。结果抗纤软肝方高、中、低剂量组小鼠ALT活性、IFN-γ和TNF-α含量均明显低于模型组,差异有显著性意义（P〈0.01）;抗纤软肝方三个剂量组肝脏病理改变明显减轻。结论抗纤软肝方对ConA所致小鼠免疫性肝损伤具有较好的保护作用,抑制T淋巴细胞活化和IFN-γ、TNF-α的释放是其主要的作用机制。

Protective effects of compound Kangxianruangan on liver injury in mice induced by concanavalin A

Objective To investigate the protective effects of Compound KangXianruangan（CK） on liver injury in mice induced by concanavalin A（Con A）.Methods Seventy-two NIH mice were randomly divided into 6 groups each consisted of 12 mice：normal control group（group A）,model group（group B）,high dosage group of CK（20.0g/kg,group C）,middle dosage group of CK（10.0g/kg,group D）,low dosage group of CK（5.0g/kg,group E）,and bifendate group（150.0mg/kg,group F）.Excepts those in group A,mice in other groups were injected with Con A（dose of 20mg/kg） via the tail vein on the first day of experiment.Group C,Group D,Group E,and Group F were given with drugs by gastric perfusion.All the drugs were given once daily for 3 succcecutive days.At the last time,4 hourse after administration of the drug,the mice were injected with Con A once again at the same dosage.The level of aminotransferase（ALT） activity,interferon-gamma（IFN-γ） and tumor necrosis factor alpha（TNF-α）content were determined 8th hour after administration of Con A.Histopathological examination was also performed for liver tissue.Results The levels of ALT activity,IFN-γ and TNF-α content in group C,D and E were obviously lower than that of group B（P〈0.01）,hepatic histopathological change were alleviated in 3 groups of CK.Conclusion CK is relatively effecive in protecting the liver injury in mice induced by Con A by suppressing the activation of the T-lymphocytes and reducing the release of IFN-γ and TNF-α.