藤梨根提取物对人胃癌BGC细胞凋亡及Bax蛋白表达的影响

目的：探讨藤梨根提取物对人胃癌BGC-823细胞凋亡的影响及其调控机制。方法：体外培养对数生长期胃癌BGC-823细胞，采用不同浓度的藤梨根提取物作用于人胃癌BGC-823细胞，流式细胞仪检测细胞凋亡，采用免疫组织化学方法检测Bax蛋白表达。结果：藤梨根作用于BGC-823，在8～32mg·mL^-1时呈不同程度的促进凋亡；藤梨根提取物大、中、小剂量组对人胃癌BGC-823细胞中Bax表达水平上调。结论：藤梨根提取物对人胃癌BGC-823细胞的增殖具有明显抑制作用，在实验浓度范围内呈浓度依赖性促进细胞凋亡，这可能与激活Bax蛋白表达有关。

The Effects of Kiwi Root Extract on Human Gastric Cancer BGC Cell Apoptosis and Bax Protein Expression

Objective：To discuss the effects of Kiwi root extract on human gastric cancer BGC cell apoptosis and its regulatory mechanism. Methods：The gastric cancer BGC-823 cells in logarithmic phase were cultivated in vitro. Kiwi root extract with different concentrations act on gastric cancer BGC-823 cells. The cell apoptosis was detected by Flow cytometry instrument, and Bax protein expression was tested by immunohistochemical methods. Results： Kiwi root can act on BGC-823 cells. Kiwi root in 8- 32mgoml^- 1 can promote cell apoptosis in the different level. Kiwi root extract raised Bax expression levels of human gastric cancer BGC- 823 cells in large dose group, medium dose group or small dose group. Conclusion.. Kiwi root extract has obvious inhibitory effecton human gastric cancer BGC-823 cells proliferation. Kiwi root extract in the experimental concentration range can promote cell ap optosis in concentration dependence, which may be related to activation of Bax protein expression.