针刺督脉穴对帕金森病小鼠多巴胺能神经元保护及超微结构的影响

目的探讨针刺督脉穴治疗帕金森病(PD)的疗效和机制。方法C57BL/6雄性小鼠随机分为5组:正常组、模型组、美多巴组、针刺组和针刺加美多巴组。造模后动物存活28d。采用爬杆法测量小鼠运动协调情况;高效液相电化学内标法测定前脑多巴胺和其代谢产物的含量;免疫组织化学技术检测中脑黑质酪氨酸羟化酶阳性神经元数目;透射电镜观察黑质神经元的超微结构变化。结果3个治疗组小鼠爬杆时间较模型组明显缩短(P<0.05),其中针刺加美多巴组较单独治疗的针刺组和美多巴组所用时间都短(P<0.05);针刺加美多巴组前脑二羟基苯乙酸(DOPAC)的含量较针刺组明显增多(P<0.05)。针刺加美多巴组的黑质酪氨酸羟化酶阳性神经元细胞数目明显高于美多巴组(P<0.05);电镜下针刺组和针刺加美多巴组黑质神经元中线粒体等结构的损伤程度也低于模型组和美多巴组。结论针刺督脉穴和针刺加美多巴复合治疗可改善PD小鼠的行为异常,其治疗PD的机制可能与减少黑质多巴胺能神经元的丢失及减轻线粒体等的损伤有关。

Protective effect of acupuncture in acupoints of Governor Vessel on dopaminergic neurons protection and influence on ultrastructure in mice with Parkinson's disease

Objective To investigate the curative effect and mechanism of acupuncture in acupoints of Governor Vessel for treating Parkinson's disease (PD).Methods All male C57BL/6 mice were randomly divided into 5 groups,including the normal group,model group,madopar group,acupuncture group and acupuncture combining with madopar group (acu+mad group).The model was established and mice survived for 28 days.The mice motor coordination was measured by using pole test.The content of dopamine and its metabolic products in ...