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| 反相高效液相色谱法同时测定人血浆中雷贝拉唑及其代谢产物 | |
| 引用本文： | 牛春燕,王嗣岑,雷莉,罗金燕,贺浪冲.反相高效液相色谱法同时测定人血浆中雷贝拉唑及其代谢产物[J].中国药房,2005,16(14):1079-1080. |
| 作者姓名： | 牛春燕 王嗣岑 雷莉 罗金燕 贺浪冲 |
| 作者单位： | 1. 新疆医科大学附属第一医院,乌鲁木齐市,830054 2. 西安交通大学医学院药事管理研究室,西安市,710061 3. 北京军区总医院,北京市,100700 4. 西安交通大学第二医院,西安市,710004 |
| 摘 要： | 目的:建立以反相高效液相色谱法同时测定人血浆中雷贝拉唑及其代谢产物硫醚雷贝拉唑和去甲基硫醚雷贝拉唑含量的方法。方法:血浆样品用冰醋酸-乙酸乙酯提取。以DiamonsilC18为反相柱,流动相为甲醇-水-冰醋酸-三乙胺(40∶60∶0.4∶0.1),流速为1.0ml/min,紫外检测波长为288nm。结果:雷贝拉唑、硫醚雷贝拉唑、去甲基硫醚雷贝拉唑各色谱峰分离良好,平均回收率分别为110.40%、87.28%、98.21%。结论:本方法灵敏度及准确度均高,可满足雷贝拉唑药动学研究的需要。 |
| 关 键 词： | 雷贝拉唑 硫醚雷贝拉唑 去甲基硫醚雷贝拉唑 反相高效液相色谱法 血浆 |
| 文章编号： | 1001-0408(2005)14-1079-02 |
| 修稿时间： | 2004年10月18 |
Simultaneous Determination of Plasma Rabeprazole and It's Metabolites by RP- HPLC |
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| NIU Chunyan,Wang Sicen,LEI Li,Luo Jinyan,HE Langchong.Simultaneous Determination of Plasma Rabeprazole and It''''s Metabolites by RP- HPLC[J].China Pharmacy,2005,16(14):1079-1080. | |
| Authors: | NIU Chunyan Wang Sicen LEI Li Luo Jinyan HE Langchong |
| Abstract: | OBJECTIVE:To establish RP-HPLC method for the simultaneous determination content of rabeprazole(RAB)and its two metabolites thioether-rabeprazole(TE)and demethylated thioether-rabeprazole(DMTE)in human plasma.METHODS:The plasma samples were extracted with ethyl acetate;The Diamonsil C 18 reversed-phase column was used as analytical column,the mobile phase was consisted of methanol-water-glacial acetic acid-triethylamine(40∶60∶0.4∶0.1)with a flow rate at1.0ml/min,the ultraviolet detection wavelength was288nm.RESULTS:Good separation of chromatographic peaks of RAB,TE and DMTE were manifested.The mean recovery of which were110.40%,87.28%and98.21%respectively.CONCLUSION:The method was sensitive and accurate,and it meets the requirements for the study of pharmacokinetic of rabeprazole. |
| Keywords: | Rabeprazole Thioether-rabeprazole Demethylated thioether-rabeprazole RP-HPLC Plasma |
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