PCR检测在真菌性角膜溃疡中的诊断价值

目的探讨聚合酶链反应(PCR)快速检测在真菌性角膜溃疡诊断中的价值。方法以源于医学条件致病性真菌18srRNA基因保守区的一对寡核苷酸序列为通用引物,对临床拟诊为真菌性角膜溃疡的36例标本进行PCR检测,并与培养方法进行对比。结果在400bp处出现DNA扩增带者为阳性。36份临床标本的真菌培养阳性率为58.3%,而经PCR扩增阳性率为86.1%,PCR扩增准确性为72.2%,敏感性为100.0%,特异性为33.3%。结论真菌通用引物进行PCR反应检测真菌性角膜溃疡速度快、阳性率高,有助于真菌性角膜溃疡的快速诊断。

Applied polymerase chain reaction detecting fungal corneal ulcer

Objective To establish a method for rapid detection of clinical suspect fungal corneal ulcer by polymerase chain reaction（PCR）. Methods A pair of oligonucleotide sequences, which was bases on the conserved region of 18srRNA shared by medically important conditioned fungal, was used as the general primers to amplify the DNAs from clinical suspect fungal corneal ulcer in a PCR assay, and the result was contrasted with culture. Results A 400bp specific DNA product was successfully amplified. The positive rate of fungi culture among 36 clinical specimens was 58.3 %, while that of PCR amplification was 86.1%. In addition, the accuracy of PCR method in this study was 72.2%, the sensitivity was 100%, and the specialty was 33.3 %. Conclusion PCR with the general primers is suitable for rapid detection of fhngal corneal ulcer because of quickness and high positive rate.