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| 药用金荞麦遗传多样性的RAPD分析 | |
| 引用本文： | 张春平 何平 胡世俊 张益锋 冀花存 高姗. 药用金荞麦遗传多样性的RAPD分析[J]. 中国中药杂志, 2009, 34(6): 660-663 |
| 作者姓名： | 张春平 何平 胡世俊 张益锋 冀花存 高姗 |
| 作者单位： | 1. 西南大学,生命科学学院,三峡库区生态环境教育部重点实验室,重庆市三峡库区植物生态与资源重点实验室,重庆,400715 2. 中国科学院,昆明植物研究所,云南,昆明,650204 |
| 摘 要： | 目的:对野生金荞麦进行遗传多样性研究.方法:通过RAPD技术对7个野生金荞麦居群共87个个体进行遗传多样性分析.结果:用12个随机引物共扩增出85条清晰条带,其中75条具多态性,平均多态性位点比率为88.24%,Nei's基因多样性指数H=0.310 3,Shannon多样性指数I=0.563 2,遗传分化指数G_(st)=0.192 4.结论:金荞麦种内具有较高的遗传多样性,遗传变异主要存在于居群内部,遗传多样性与地理关系表现出明显的相关性.RAPD可以作为研究遗传多样性及遗传分化的有效标记. |
| 关 键 词： | 金荞麦 遗传多样性 RAPD 聚类分析 遗传分化 |
RAPD analysis for genetic diversity of Fagopyrum cymosum |
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| ZHANG Chun-Beng-,He-Beng-,Hu-Shi-Dun-,Zhang-Yi-Feng-,Ji-Hua-Cun-,Gao-Pan-. RAPD analysis for genetic diversity of Fagopyrum cymosum[J]. China Journal of Chinese Materia Medica, 2009, 34(6): 660-663 | |
| Authors: | ZHANG Chun-Beng- He-Beng- Hu-Shi-Dun- Zhang-Yi-Feng- Ji-Hua-Cun- Gao-Pan- |
| Affiliation: | 1. School of Life Sciences, Southwest University, Key Laboratory (Ministry of Education) of Eco environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and Resources Research for Three Gorges Reservoir Region, Chongqing 400715, China; 2. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China |
| Abstract: | Objective: To study the genetic diversity of Fagopyrum cymosum. Method: The genetic diversity of 87 individuals from 10 populations was analyzed by random amplified polymorphic DNA (RAPD). Result: Twelve primers were selected to produce highly reproducible RAPD bands. Among 85 amplified bands, seventy five showed polymorphism, the percentage of polymorphic bands reached to 88.24%. Nei's gene diversity index (H) was 0.310 3, Shannon information index (I) was 0.563 2, Gst was 0.192 4. The genetic similarity coefficient and the genetic distance were 0.672 0 0.967 8 and 0.032 8 0.397 5, respectively. Conclusion: F. cymosum shows high genetic diversity and the majority of genetic variation occurs in populations. By cluster analysis, the geographical distribution is very obvious. The RAPD marker can be used for the analysis of the genetic diversity and genetic variation of F. cymosum. |
| Keywords: | Fagopyrum cymosum genetic diversity RAPD cluster analysis genetic variation |
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