古城玫瑰树中非生物碱类化学成分研究(Ⅱ)

目的研究玫瑰树属植物古城玫瑰树Ochrosia elliptica枝叶中的非生物碱类化学成分。方法综合运用硅胶柱色谱、反相硅胶柱色谱和Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等方法进行系统分离,根据理化性质及波谱学数据鉴定化合物结构。结果从古城玫瑰树90%乙醇提取物的醋酸乙酯萃取部位中分离鉴定了15个非生物碱类化合物,分别鉴定为hedyotisol A(1)、(-)-(7R,7′R,7″S,8S,8′S,8″S)-4′,4″-dihydroxy-3,3′,3″,5-tetramethoxy-7,9′,7′,9-diepoxy-4,8″-oxy-8,8′-sesquineolignan-7″,9″-diol(2)、8-羟基松脂素(3)、lyoniresinol(4)、蛇菰脂醛素(5)、刺芒柄花素(6)、vestisol(7)、3′,7-二羟基-2′,4,-二甲氧基异黄烷(8)、(3R)-violanone(9)、6-羟基-2-(2-苯乙基)色酮(10)、6-羟基-2-2-(4′-甲氧基苯基)乙基]色酮(11)、对羟基苯乙酮(12)、去氢催吐萝芙木醇(13)、黑麦草内酯(14)和4,5-dihydroblumenol A(15)。其中化合物1~5为木脂素类化合物,化合物6~9为异黄酮类化合物,化合物10和11为色原酮类化合物,化合物12为酚酸类化合物,化合物13~15为倍半萜类化合物。结论所有化合物均为首次从玫瑰树属植物中分离得到。

Studies on non-alkaloid constituents from Ochrosia elliptica(II)

Objective To study the non-alkaloid constituents from stems and leaves of Ochrosia elliptica. Methods The chemical constituents from O. elliptica were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatography, and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data in literature. Results Fifteen compounds were isolated from the ethyl acetate fraction of 90% ethanol extract from O. elliptica, which were identified as hedyotisol A (1), (-)-(7R,7''R,7"S,8S,8''S,8"S)-4'',4"-dihydroxy-3,3'',3",5-tetramethoxy-7,9'',7'',9-diepoxy-4,8"-oxy-8,8''-sesquineolignan-7",9"-diol (2), 8-hydroxypinoresinol (3), lyoniresinol (4), balanophonin (5), formononetin (6), vestitol (7), mucronulatol (8), (3R)-violanone (9), 6-hydroxy-2-(2-phenylethyl) chromone (10), 6-hydroxy-2-2-4''-(methoxyphenyl) ethyl] chromone (11), 4-hydroxyacetophenone (12), dehydrovomifoliol (13), loliolide (14), and 4,5-dihydroblumenol A (15). Conclusion All compounds are isolated from the genus Ochrosia Juss. for the first time.