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塞来昔布联合吉西他滨对Lewis肺癌皮下移植瘤的抑制作用
引用本文:姚素萍,潘晓芳,刘业六. 塞来昔布联合吉西他滨对Lewis肺癌皮下移植瘤的抑制作用[J]. 江苏医药, 2012, 38(1): 12-14,129
作者姓名:姚素萍  潘晓芳  刘业六
作者单位:1. 淮安市淮阴医院肿瘤内科,江苏省,223300
2. 南京医科大学附属淮安第一医院
摘    要:目的研究塞来昔布(CEL)与吉西他滨(GEM)联用对Lewis肺癌皮下移植瘤的抑制效应。方法 40只小鼠随机分为对照组(A组)、GEM组(B组)、CEL组(C组)和CEL/GEM联用组(D组),每组10只。A、B组给予普通饲料;C、D组自接种后第2天给予含CEL 1mg/g的饲料喂养。B、D组于肿瘤接种后第7天,给予GEM 100mg/kg溶液0.2ml腹腔注射;A、C组给予等量生理盐水。每周注射1次,连续3周。实验期间测量肿瘤长短径,并绘制肿瘤生长曲线,4周后处死,称量瘤重,计算抑瘤率;检测移植瘤组织血管内皮生长因子(VEGF)表达、微血管密度(MVD)和肿瘤细胞的凋亡。结果 B、C和D组抑瘤率分别为54.18%、49.52%和88.59%;D组移植瘤组织的VEGF表达及MVD低于A、B和C组(P<0.05),而凋亡指数高于A、B和C组(P<0.05)。结论 CEL和GEM均能抑制Lewis肺癌移植瘤的生长,两者联用通过抑制肿瘤微血管生成及诱导肿瘤细胞凋亡发挥协同抗肿瘤效应。

关 键 词:Lewis肺癌  塞来昔布  吉西他滨

Inhibitory effects of celecoxib combined with gemcitabine on subcutaneously xenografs of Lewis lung carcinoma
YAO Suping , PAN Xiaofang , LIU Yeliu. Inhibitory effects of celecoxib combined with gemcitabine on subcutaneously xenografs of Lewis lung carcinoma[J]. Jiangsu Medical Journal, 2012, 38(1): 12-14,129
Authors:YAO Suping    PAN Xiaofang    LIU Yeliu
Affiliation:. Department of Oncology,Huaiyin Hospital of Huaian,Huaian 223300, CHINA
Abstract:Objective To study the inhibitory effects of celecoxib combined with gemcitabine on subcutaneously xenografs of Lewis lung carcinoma(LLC). Methods Forty mice were equally randomized into 4 groups of A(control), B(treated with gemcitabine),C(treated with celecoxib) and D(treated with celecoxib and gemcitabine) with 10 mice each.The rats in groups of A and B were given normal food and those in groups of C and D were given the diet with celecoxib 1mg/g starting on the next day after implantation.The rats in groups of B and D were given gemcitabine 100mg/kg(0.2ml) intraperitoneally injection weekly since the 7th day after implantation and those in groups of A and C were given equal volume of normal saline.All mice were sacrificed 4 weeks later.During the experiment,growth of tumor was calculated by measuring the long and short diameters and tumor growth curve was delineated.Tumors were weighed and the inhibition rates were obtained.The expressions of VEGF,MVD and apoptosis index(AI) of tumor samples were detected. ResultsCompared with group A,the tumor growth was significantly inhibited in groups of B,C and D with the corresponding inhibition rates of 54.18%,49.52% and 88.59%, respectively.The expressions of VEGF and MVD were lower,but AI was higher,in group D than those in groups of A,B and C. Conclusion Celecoxib and gemcitabine could inhibit the growth of xenografs of LLC.Combined use of celecoxib and gemcitabine has a synergistic antitumor effect by angiogenesis inhibition and apoptosis induction of LLC.
Keywords:Lewis lung carcinoma  Celecoxib  Gemcitabine
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