A simple fluorometric assay for quantifying the adhesion of tumour cells to endothelial monolayers |
| |
| Authors: | Elizabeth A. Price Deirdre R. Coombe J. Clifford Murray |
| |
| Affiliation: | (1) CRC Academic Department of Clinical Oncology, City Hospital, University of Nottingham Laboratory of Molecular Oncology, Nottingham, UK;(2) Institute for Child Health Research, West Perth, Australia;(3) CRC Academic Dept. of Clinical Oncology, City Hospital, University of Nottingham Lab. of Molecular Oncology, Hucknall Road, Nottingham, NG5 1PB, UK |
| |
| Abstract: | ![]()
A static adhesion assay employing 6-carboxy-3 ,6-diacetylfluorescein (6-CFDA) as a fluorescent marker has been developed to study the interactions of tumour cell lines with endothelial monolayers. This assay allows simple, safe quantification of cell-cell adhesion using living cells. It has been used to demonstrate that the integrin adhesion molecule VLA-4 mediates the attachment of RPMI-7951 melanoma cells to human umbilical vein endothelial cells (HUVEC) which have been activated by TNF . In addition, MDA-MB-231 breast adenocarcinoma cells display greater adhesion to microvessel endothelial cells than to large vessel endothelial cells. |
| |
| Keywords: | adhesion carcinoma endothelium melanoma metastasis |
| 本文献已被 SpringerLink 等数据库收录！ |
|
