CD4＋CD25＋FOXP3＋调节性T细胞和PD-1＋CD4＋T细胞对宫颈内皮细胞瘤预后的作用

目的：研究宫颈内皮细胞瘤（CIN）皮损处的CD4＋ CD25＋ FOXP3＋调节性T细胞（Tregs）和 PD‐1＋ CD4＋ T细胞及其转归之间的关联性。方法用细胞刷从CIN患者收集宫颈淋巴细胞，并用 FACS分析这些细胞中的CD4＋ CD25＋ FOXP3＋T regs和PD‐1＋CD4＋ T细胞。比较CIN消退者和未消退者之间CD4＋ CD25＋ FOXP3＋ T regs和 PD‐1＋ CD4＋ T 细胞群的差异。结果宫颈淋巴细胞中CD4＋CD25＋ FOXP3＋ T regs占11．8％，而PD‐1＋CD4＋ T细胞占30．3％。与未消退者组比较，CIN消退组CD4＋CD25＋ FOXP3＋ Tregs和 PD‐1＋ CD4＋ T 细胞均明显较低（P＜0．05）。结论宫颈 CD4＋ CD25＋ FOXP3＋ Tregs的产生与CIN自发消退成负相关，表明Tregs在人乳头瘤状病毒（HPV）相关的肿瘤免疫逃逸中起重要作用。

The roles of CD4+CD25+ FOXP3+ regulatory T cells and PD-1+CD4+T cells on prognosis of cervical intraepithelial lesion

Objective This study aims to investigate the correlation of CD4+ CD25+ FOXP3+ Tregs and PD‐1+ CD4+ T cells located at cervical lesions and the prognosis of CIN .PD‐1 expression on T cells induces Tregs .Methods Cervical lymphocytes were collected from CIN patients and analyzed by FACS .Comparisons were made between populations of cervical Tregs and PD‐1+CD4+T cells in CIN regressors and non‐regressors .Results A median of 11 .8% of cervical CD4+ T cells were CD4+ CD25+ FOXP3+Tregs ,while a median of 30 .3% were PD‐1+ CD4+ T cells .The proportions of CD4+ CD25+ FOXP3+ Tregs and PD‐1+ cells were significantly lower in CIN regressors when compared with non‐regressors .Conclusion The prevalence of CD4+ CD25+ FOXP3+Tregs negative correlates with spontaneous regression of CIN ,suggesting that cervical Tregs may play an important role in HPV‐related neoplastic immunoevasion .