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| HPLC法测定胸腺五肽的含量 | |
| 引用本文： | 刘璐,宋月英,张庆云,张莉. HPLC法测定胸腺五肽的含量[J]. 中国药房, 2007, 18(28): 2210-2211 |
| 作者姓名： | 刘璐 宋月英 张庆云 张莉 |
| 作者单位： | 中国人民武装警察部队医学院,天津市,300162 |
| 基金项目： | 天津市武警医学院青年基金 |
| 摘 要： | 目的:建立以高效液相色谱法测定胸腺五肽(TP-5)含量的方法。方法:色谱柱为Kromasil C18,流动相为12%乙腈(含0.1%三氟乙酸),检测波长为222nm,流速为1.5mL.min-1,柱温为30℃,进样量为20μL。结果:TP-5检测浓度的线性范围为100～400μg.mL-1(r=0.9998);平均回收率为100.61%,RSD=0.46%。结论:本方法操作简便、灵敏、准确,可用于TP-5的含量检测。 |
| 关 键 词： | 高效液相色谱法 胸腺五肽 含量测定 |
| 文章编号： | 1001-0408（2007）28-2210-02 |
| 收稿时间： | 2006-11-09 |
| 修稿时间： | 2007-07-28 |
Determination of Thymopentin by HPLC |
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| LIU Lu,SONG Yueying,ZHANG Qingyun,ZHANG Li. Determination of Thymopentin by HPLC[J]. China Pharmacy, 2007, 18(28): 2210-2211 | |
| Authors: | LIU Lu SONG Yueying ZHANG Qingyun ZHANG Li |
| Abstract: | OBJECTIVE:To develop a HPLC method for the determination of the content of thymopentin(TP-5).METHODS:Thymopentin was separated on Kromasil C18 with column temperature set at 30℃.The mobile phase consisted of 12% acetonitrile(containing 0.1% trifluoroacetic acid)with flow rate of 1.5mL· min-1.The wavelength was set at 222nm and the sample size was 20μ L.RESULTS:The standard curve of thymopentin was linear within the range 100～ 400μ g· mL-1(r=0.999 8),with average recovery at 100.61% and precision at 0.46%.CONCLUSION:This method is convenient,sensitive and accurate,and it can be used for determination of thymopentin. |
| Keywords: | HPLC Thymopentin Content determination |
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