白纹伊蚊和致倦库蚊Wolbachia 16S rRNA序列测定与分析

【目的】PCR法扩增与测定白纹伊蚊和致倦库蚊的细胞内共生菌 Wolbachia16SrRNA序列 ,分析其种系发生关系及传播方式。【方法】从单只雌蚊中提取DNA ,PCR法扩增 16SrRNA序列 ,扩增产物进行克隆及测序。【结果】从白纹伊蚊和致倦库蚊分别扩增出Wolbachia 16SrRNA序列 ,并克隆入 pGEM T载体 ,测序证明两序列长度分别为 897bp。【结论】成功克隆了白纹伊蚊和致倦库蚊Wolbachia 16SrRNA序列。测序和同源性分析表明 ,它们之间的同源性为 99 9%,提示Wolbachia在白纹伊蚊和致倦库蚊可能存在水平传播的方式。

Identification and Analysis of Wolbachia 16S rRNA from Aedes albopictus and Culex pipiens quinquefasciatus

Objective To amplify and analyze the Wolbachia 16S rRNA sequences from Aedes albopictus and Culex pipiens quinquefasciatus, and to analyze their phylogenetic relationship. Methods DNA was extracted from single female mosquito. Wolbachia 16S rRNA sequence was ampified by PCR method. The amplification product was cloned and identified by sequencing. Results Wolbachia 16S rRNA sequences were obtained from Aedes albopictus and Culex pipiens quinquefasciatus. The two sequences were cloned into pGEM-T vector. The sequencing result shows they have 897 bp in length. Conclusion Wolbachia 16S rRNA of Aedes albopictus and Culex pipiens quinquefasciatus were cloned successfully. The result of sequencing and homology analysis testifies their homology is 99.9%. It suggests that Wolbachia may have a possible horizontal transmission mechanism.