急性脑梗死患者血清sFas和sFasL水平的观察研究

目的 通过检测急性脑梗死(acute cerebral infarction,ACI)患者血清可溶性Fas(soluble Fas,sFas)和可溶性Fas配体(soluble Fasligand,sFasL)表达水平的变化,探讨sFas和sFasL与ACI病情变化的关系.方法 60例ACI患者(男性32例,女性28例)为研究组,30例健康体检者(男性12例,女性18例)为对照组.用酶联免疫吸附法检测两组血清sFas和sFasL水平,比较两组之间sFas和sFasL浓度的差异.结果 ACI组48 h、7 d和14 d时血清sFas水平分别为(6.27±1.48)ng/L、(4.99±1.15)ng/L和(3.74±0.58)ng/L,均显著高于对照组的(3.00±0.38)ng/L(F=7.29,P<0.01);ACI组48 h、7 d和14 d时血清sFasL水平分别为(4.40±1.32)ng/L、(3.19±0.94)ng/L和(1.91±0.45)ng/L,均显著高于对照组的(1.15±0.21)ng/L(F=8.60,P<0.01).大梗死组sFas和sFasL水平分别为(7.63±0.64)ng/L和(5.01±1.16)ng/L,显著高于小梗死组的(4.98±0.91)ng/L(t=12.12,P<0.01)和(3.58±0.87)ng/L(t=5.35,P<0.01).ACI患者血清sFas和sFasL水平呈正相关性(r=0.748,P=0.01).结论 血清sFas和sFasL水平高提示ACI患者梗死体积较大.

The levels of serum sFas and sFasL in patients with acute cerebral infarction: an observational study

Objective To investigate the changes of serum soluble Fas (sFas) and soluble Fas-ligand (sFasL), and the relationship betweenthe level of serum sFas or sFasL and the infarct volume in patients with acute cerebral infarction (ACI). Methods Sixty patients with ACI (female 28, male 32) served as study group and 30 healthy subjects (female 18, male 12) served as control group. An enzyme-linked immunosorbent assay was used to detect the levels of serum sFas and sFasL in both groups, and the differences of the sFas and sFasL concentration were compared between the two groups. Results The levels of serum sFas at 48 hours, at day 7 and 14 in the ACl group were 6. 27 ± 1.48 ng/L, 4. 99 ± 1.15 ng/L, and 3.74 ± 0.58 ng/L,respectively, and they were all significantly higher than 3.00 ± 0. 38 ng/L in the control group (P <0. 05). The levels of serum sFasL at 48 hours, at day 7 and 14 in the ACI group were 4.40 ± 1.32 ng/L, 3. 19 ± 0.94 ng/L, and 1.91±0.45 ng/L, respectively. They were significantly higher than 1.15 ±0.21 ng/L in the control group (P<0.01). The levels of sFas (1.91 ± 0.45) ng/L, respectively, and they were all significantly higher than (4.98 ±0.91) ng/L(t = 12.12 ,P <0. 01)and (3.58 ±0. 87) ng/L(t =5.35 ,P <0.01) in the small infarction group. The levels of serum sFas and sFasL in patients with ACI showed positive correlation (r =0. 748, P =0. 01). Conclusions High serum sFas and sFasL may indicate larger infarct volume in patients with ACI.