血管内皮生长因子C在宫颈癌中的表达及其与淋巴转移的关系

目的分析正常宫颈，宫颈上皮内瘤样病变，无淋巴结转移，有淋巴结转移组的宫颈鳞癌组织中VEGF-C的表达。方法收集84例正常宫颈组织，宫颈上皮内瘤样病变和宫颈鳞癌组织（按有无淋巴转移、分化及临床分期分组）。进行SP免疫组化染色，测定VEGF-C蛋白的表达。结果VEGF-C表达主要位于肿瘤细胞胞浆及胞膜内。它在正常宫颈、CIN及宫颈鳞癌组织中的表达依次增高，有淋巴结转移组较无淋巴结转移组中VEGF-C的表达显著增高。VEGF-C的表达与患者的临床分期无关（P〉0．05），与富颈癌的病理分级呈负相关。结论提示VEGF-C有促进宫颈癌发展、促进淋巴管生成、抑制癌细胞分化的作用。由于VEGF-C与淋巴道转移的密切关系，故可在临床工作中通过检测宫颈癌活检组织中VEGF-C的表达估计其预后，为宫颈癌的治疗提供新策略。

Expression of VEGF-C in Cervical Carcinoma and itsRelation to Lymphatic Metastasis

Objective To study the expression of vascular endothelial growth factor-C(VEGF-C) in normal uterial cervix,cervical intraepithelial neoplasia(CIN)and carcinoma of cervix uteri with or without lymphatic metastasis.Methods We collected 84 samples including normal uterial cervix tissues,cervical intraepithelial neoplasia(CIN) tissues and carcinoma of cervix uteri tissues.VEGF-C was stained immunohistochemically.Results VEGF-C was detected mainly in cytoplasm or cytomembrane of tumor cell.The expression rates of VEGF-C were gradually increased with the progress cervical lesions.The expression of VEGF-C was higher in cervical cancer with lymphatic metastasis than that without lymphatic metastasis.There did not exsist correlation between the expression of VEGF-C and clinical stage.The expression of VEGF-C had negative correlation with the pathologic grade of cervical carcinoma.The expression of VEGF-C in low differentiation group was significantly higher than that in middle/high differentiation groups.Conclusion The results suggested that VEGF-C could accelerate the development of cervical carcinoma,promote the generation of lymphatic vessel,so that accelerate the metastisis and invasion of cervical carcinoma,suppress the differentiation of cancer.Considering the close relationship between the expression of VEGF-C and lymphatic metastisis,examining the expression of VEGF-C in the biopsy tissues of cervical carcinoma could be applied in clinical work to estimate the prognosis of cancer,and provide the therapy with new tactics.