用扫描仪评价不同产地赤芍提取物对红细胞聚集的抑制作用

为了解不同产地赤芍乙醇提取物对于红细胞聚集的影响 ,本文采用扫描仪对此进行了评价。用葡聚糖 5 0 0诱导大鼠红细胞在体外悬浮液中的聚集 ,观察终浓度 138mg/ml的赤芍提取物对此的影响。实验表明 ,细胞悬浮液扫描图象的灰度与红细胞悬浮液的细胞聚集程度有显著相关。实验结果表明 ,六个产地中的四个赤芍提取物对于红细胞聚集有明显抑制 ,其中内蒙古多伦出产的赤芍效果最明显 (P <0 0 0 1)。结果可说明药材的道地性

Inhibition on Scanning Evaluated Erythrocyte Aggregation by Paeonia Extracts

The alcohol extracts of Paeonia laciflora Pall from six origins were prepared for evalution of efficacy on inhibition of rat erythrocyte aggregation.In vitro erghrocyte aggregation in suspension was induced by Dextran 500. The final concentration of Paeonia extracts was controlled at 138 mg(crude herb)/ml. Erythrocyte aggregation was evaluated by optical transmission of erythrocyte suspensions in a scanner.The result showed that gray scale was correlated with degree of erythrocyte aggregation significantly.Four extracts out of the tested six significantly inhibited erythrocyte aggregation indicated by the diminished values in gray scale.In fact,the authentic Paeonia lactiflora Pall produced in Duolun,Inner Mongolia,demonstrated the most significant effect (P<0.001).