抗凋亡基因bcl-xL与白血病细胞耐药的相关性研究

目的 研究ｂｃｌ－ＸＬ基因高表达的白血病细胞耐药形成中的作用,观察急性髓细胞白血病病人的ｂｃｌ－ＸＬ表达与临床疗效间的相关性。方法 采用脂质体法将ｂｃｌ－ＸＬ ｃＤＮＡ转导入ＨＬ－６０细胞;流式细胞仪定量检测凋亡细胞：ＭＴＴ法测定足叶乙甙、柔红霉素、阿霉素对转染细胞的细胞毒性;ＲＴ－ＰＣＲ法检测２０例急性髓细胞白血病病人的ｂｃｌ－ＸＬ表达。结果 ｂｃｌ－ＸＬ高表达可抑制足叶乙甙诱发的凋亡,抑制率为

Relationship of anti-apoptosis gene bcl-xL and multidrug resistance in leukemia cells

Objective: To investigate the role of bcl-XL gene in development of multidrug resistance and to observe the relationship between the expression of bcl-X_L in acute myeloid leukemia and clinical response. Methods: bcl-X_L. eDNA was transfected into HL-60 cells using lipofictamine. The apoptotic rate was quantitatively analyzed with FCM. The cytotoxicity of chemotherapy drugs in vitro was compared employing the MTT assay. RT-PCR was applied to detect the expression of bcl-X_L. in 20 patients with acute myeloid leukemia. Results: The overexpression of bcl-XL in HL-60 cells inhibited the etoposide-induced apoptosis by 40. 5% and decreased the cytotoxicity of etoposide. daunorubicin and adriamycin. The resistance factors were 3. 2, 3. 2, 1. 6, respectively. Sixteen of 20 patients with acute myeloid leukemia expressed bcl-X_L mRNA. Only 2/7 (28. 6% )cases with higher level of bcl-X_L mRNA achieved complete remission, while 6/9 (66. 8% ) cases with lower level of bcl-X_L mRNA got complete remission. Conclusion: bcl-X_L gene might he involved a new multidrug resistance phenotype through inhibiting the apoptosis induced by chemotherapeutic agents. The detection of bcl-X_L gene also provides a new parameter to predict The prognosis of acute myeloid leukemia.