Alteration of antigenic expression of human myeloid precursor cells (CFU-GM) in long-term cultures |
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| Authors: | Z Williams E Gazit I Fabian |
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| Affiliation: | 1. Central Japan Cord Blood Bank, Seto, Japan;2. Nakagami Hospital, Okinawa, Japan;3. Japanese Red Cross Kanto-Koshinetsu Block Blood Center, Tokyo, Japan;4. Hyogo Cord Blood Bank, Kobe, Japan;5. Japanese Red Cross Blood Service Headquarters, Tokyo, Japan;6. Japanese Red Cross Kinki Block Blood Center, Ibaraki, Japan;7. Japanese Red Cross Kyushu Block Blood Center, Chikushino, Japan;8. Japanese Red Cross Hokkaido Block Blood Center, Sapporo Japan;9. Department of Hematology, Federation of National Public Service Personnel Mutual Aid Associations Toranomon Hospital, Tokyo Japan;10. Division of Clinical Precision Research Platform, The Institute of Medical Science, the University of Tokyo, Tokyo, Japan;11. Department of Hematology, Kanagawa Cancer Center, Yokohama, Japan;12. Hematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Tokyo, Japan;13. Department of Hematology, Tokyo Metropolitan Institute for Geriatrics and Gerontology, Tokyo, Japan;14. Tokyo Metropolitan Tama Medical Center, Fuchu, Japan;15. Department of Hematology/Oncology, Tokai University School of Medicine, Isehara, Japan;16. Department of Hematology, Hamanomachi Hospital, Fukuoka, Japan;17. Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto, Japan;18. Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan;19. Japanese Data Center for Hematopoietic Cell Transplantation, Nagakute, Japan;20. Department of Registry Science for Transplantation, Aichi Medical University School of Medicine, Nagakute, Japan;1. Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto, Japan;2. Center for Research and Application of Cellular Therapy, Kyoto University Hospital, Kyoto, Japan;3. Department of Hematology, Sakai City Medical Center, Osaka, Japan;4. Japanese Red Cross Kinki Block Blood Center, Osaka, Japan;5. Japanese Red Cross Blood Service Headquarters, Tokyo, Japan |
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| Abstract: | ![]()
Comparison of myelopoiesis in continuous human bone marrow and cord blood cultures was performed. The presence of major histocompatibility complex (MHC) antigens on myeloid precursors (CFU-GM) from cord blood was confirmed using monoclonal antibodies (MoAb) defining class I (i.e., anti-beta 2-microglobulin (B2, MG1) anti-HLA-A,B heavy chain (W6/32), and anti-HLA locus B(4E), and class II (HLA-DR (7,2] antigens. The effect of long-term culture on the expression of these antigens was investigated. Our studies indicate that following prolonged in vitro culture there is a decrease in the expression of MHC antigens on CFU-GM. Our observations provide a rationale for considering the use of cord blood leukocytes subjected to long-term culture as an alternative to allogeneic hematopoietic cell transplantation. |
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