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| p38 MAPK在LPS诱导牙龈成纤维细胞表达uPA中作用的研究 | |
| 引用本文： | 陈健,牛忠英,药立波. p38 MAPK在LPS诱导牙龈成纤维细胞表达uPA中作用的研究[J]. 牙体牙髓牙周病学杂志, 2002, 12(9): 466-469 |
| 作者姓名： | 陈健 牛忠英 药立波 |
| 作者单位： | 1. 第四军医大学口腔医学院,陕西,西安,710032 2. 解放军306医院,北京,100101 3. 第四军医大学生物化学与分子生物学教研室,陕西,西安,710032 |
| 摘 要： | 目的：研究p38 MAPK在LPS诱导牙龈成纤维细胞表达uPA中的作用。方法：采用Western blotting观察LPS对牙龈成纤维细胞内p38 MAPK活性的影响；蛋白激酶活性实验SB203580地p38 MAPK活性的抑制作用；Northern blotting观察SB203580对LPS诱导uPA表达的影响。结果：LPS能够迅速地激活牙龈成纤维细胞内p38 MAPK的活性；SB203580能够有效地抑制牙龈成纤维细胞内的p38 MAPK的活性；经SB203580处理后，LPS对uPA的诱导作用受到显著的抑制。结论：LPS通过p38 MAPK信号转导途径诱导牙龈成纤维细胞表达uPA。 |
| 关 键 词： | 牙龈成纤维细胞 内毒素脂多糖 尿激酶型纤溶酶原激活剂 p38 MAPK |
| 文章编号： | 1005-2593(2002)09-0466-04 |
| 修稿时间： | 2002-05-28 |
Lipopolysaccharide induces uPA expression by human gingival fibroblasts via p38 MAPK pathway |
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| CHEN Jian,NIU Zhong-ying,YAO Li-bo. Lipopolysaccharide induces uPA expression by human gingival fibroblasts via p38 MAPK pathway[J]. Chinese Journal of Conservative Dentistry, 2002, 12(9): 466-469 | |
| Authors: | CHEN Jian NIU Zhong-ying YAO Li-bo |
| Abstract: | AIM:To investigate the role of p38 MAPK in LPS-induced uPA expression by human gingival fibroblasts. METHODS:Activity of p38 MAPK in human gingival fibroblasts induced by LPS was analyzed by western blotting. Inhibitory effect of SB203580 on p38 MAPK was measured by MAPKAPK-2 kinase assay. Effect of SB203580 on uPA expression by human gingival fibroblasts induced by LPS was observed by Northern blotting.RESULTS:LPS activated p38 MAPK in human gingival fibroblasts. SB203580 inhibited p38 MAPK activity significantly. SB203580 inhibited LPS-induced uPA expression by human gingival fibroblasts. CONCLUSION:p38 MAPK activity is required for LPS-induced uPA expression in human gingival fibroblasts. |
| Keywords: | human gingival fibroblasts lipopolysaccharide(LPS) urokinase-type plasminogen activator(uPA) p38 MAPK |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |