拔牙位点保存用复合温敏凝胶的构建及体外表征

目的:制备多壁碳纳米管/羟基磷灰石/壳聚糖复合温敏凝胶,探讨其作为拔牙位点保存材料的理化性能。方法:采用原位沉积方法制备多壁碳纳米管/羟基磷灰石(MWNT/ HA简称HAC)复合无机材料,将其分散至壳聚糖温敏凝胶中,制备壳聚糖/多壁碳纳米管/羟基磷灰石(CS /MWNT/HA简称CS/HAC)复合温敏凝胶,以成胶时间、孔径、孔隙率、机械强度及降解性能为指标优化配方设计,并表征复合温敏凝胶的微观形貌及理化性能。结果:以10 g/L的HAC作为补强成分加入到壳聚糖溶液中,能够在不影响成胶并保证一定孔隙率(84%)的前提下,提高材料的机械性能(抗压强度0.441 MPa),所获复合温敏凝胶具有适宜的临床操作时间(12 min),并具有可控的降解速率。结论:负载HAC的复合温敏凝胶具有良好的成型性,并具有较好的机械性能,在拔牙位点保存领域具有良好的应用前景。

Preparation and Characterization of Composite Thermo-sensitive Gel for Alveolar Ridge Preservation.

Objective: To develop multi-walled carbon nanotube/nano-hydroxyapatite/chitosan(CS)/β-glycerophosphate(GP) injectable thermo-sensitive gels and to explore its feasibility as alveolar ridge preservation material. Methods: Wet-chemical in-situ precipitation route was used to obtain CNT/HA inorganic composites (HAC), which were dispersed in chitosan solution to obtain multi-walled carbon nanotube/nano-hydroxyapatite/chitosan thermo-sensitive gels. Gelation time, hole size, the porosity, mechanical strength and the percentage weight loss were selected as indicators to optimize the matching. The microstructure and physicochemical properties of the thermo-sensitive gels were characterized. Results: The results showed that the gel-forming property, including certain porosity (84%) and improved mechanical properties (0.441MPa), could be well tuned by controlling the amount of HAC (10 mg/mL) composite incorporated. In addition, certain amounts of HAC made the degradation rate controllable and shorten the clinical operation time to 12 min. Conclusion: The HAC loaded CS/GP composite scaffolds developed in this study exhibited good formability and mechanical properties and it might be a promising selection for bone tissue engineering.