Adult peripheral lung organ culture--a model for respiratory tract toxicology |
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| Authors: | M E Placke G L Fisher |
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| Affiliation: | 1. State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (School of Chemistry and Pharmacy, Guangxi Normal University), Guilin 541004, PR China;2. School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530008, PR China;3. Guangxi Colleges and Universities Key Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi, College of Chemistry and Environmental Engineering, Baise University, Baise, Guangxi 533000, PR China;1. Institut Universitaire de France IUF, 103 Boulevard St Michel, Paris F-75005, France;2. Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Institut Lumière Matière, F-69622 Villeurbanne, France;3. Université Paris-Saclay, CNRS, CEA, Univ Evry, Laboratoire Analyse et Modélisation pour la Biologie et l’Environnement, F-91025 Evry, France;1. School of Pharmaceutical Sciences, Shandong University, Jinan, China;2. Weihai International Biotechnology Research and Development Centre, Shandong University, Weihai, China;1. University of Liège—Gembloux Agro-Bio Tech, Unit of Biological and Industrial Chemistry (CBI), 2, Passage des déportés, 5030 Gembloux, Belgium;2. Yale University—Center for Green Chemistry and Green Engineering, 370 Prospect Street, Greeley Memorial Lab. 2A, New Haven, CT 06511, USA;3. University of Liège—Gembloux Agro-Bio Tech, Food Science and Formulation Department (SAF), 2, Passage des déportés, 5030 Gembloux, Belgium |
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| Abstract: | ![]()
This report describes procedures to culture 1- to 2-mm-thick cross sections of lung lobes for periods of 4 to 6 weeks. Normal morphologic and macromolecular composition are maintained. Previous attempts to maintain adult peripheral lung cultures for periods beyond 7-10 days or to examine respiratory disorders in vitro other than acute changes have been generally unsuccessful. Eight different, supplemented, serum-free media, mixed with heated liquid agarose were infused into the airways of hamster and rat lungs. Cross sections were explanted onto squares of porous surgical packing material, placed in medium, and incubated for 4 to 6 weeks. The ability of each medium to maintain normal lung was assessed microscopically by quantitative image analysis and by biochemical analyses. The optimal medium formulation for each species is described. The adult peripheral lung culture system may provide toxicologists with a unique model for mechanistic and safety evaluations of potential lung toxicants. |
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