宫颈上皮内瘤变的HPV感染及P53和MDM2的表达

目的研究宫颈上皮内瘤(CIN)的人类乳头状瘤病毒(HPV)感染、p53蛋白和MDM2蛋白的表达及三者之间的相互关系。方法选取CIN 47例、正常宫颈组织54例,分别采用原位杂交技术检测HPV6/11、HPV16/18感染,用免疫组织化学技术(SP法)检测P53蛋白和MDM2蛋白。结果HPV6/11感染率在CIN组和对照组分别为46.8%(22/47)、20.4%(11/54),HPV18感染分别为70.2%(33/47)、16.7(9/54)。CIN组的HPV6/11、HPV16/18的感染率均显著高于对照组(均P<0.01)。CIN组和对照组的p53阳性率分别为2.27%(13/47)、3.7%(2/54)。CIN组和对照组的MDM2阳性率分别为14.9%(7/47)、7.4%(4/54)。CIN组的p53阳性率显著高于对照组(P<0.05),MDM2阳性率CIN组和对照组之间无统计学差异(P>0.05)。在本实验中,p53、MDM2之间的阳性率均无统计学相关(P>0.05)。p53、MDM2蛋白阳性表达和HPV6/11、HPV16/18感染之间无统计学相关(均P>0.05)。结论CIN与HPV感染关系密切;p53可能参与小部分CIN的发生发展,而MDM2可能在CIN的发生发展中不起重要作用。

Relationships of Human Papillomavirus Infection and Expression of P53, MDM2 proteins in Cervical Intra- epithelial Neoplasia

Objective To investigate Human Papillomavirus(HPV) infection and expression of P53,MDM2 proteins,as well as their relationships in cervical intraepithelial neoplasia(CIN).Methods 47 cases of cervical intraepithelial neoplasia and 54 controls with normal cervix were selected.HPV6/11 and HPV16/18 infection was detected using in site hybridization method,and expression of P53 and MDM2 proteins was examined by immunohistochemistry streptavidin peroxidase conjugated method.Results The infection rates of HPV6/11 in CIN and control groups were 46.8%(22/47) and 20.4%(11/54) respectively,while those of HPV16/18 were 70.2%(33/47) and 16.7%(9/54) respectively.HPV6/11 and HPV16/18 infection rates of CIN group were significantly higher than those of control group(P<0.01). The P53 protein positive rates in CIN and control groups were 27.7%(13/47) and 3.7%(3/54) respectively and had significant difference(P<0.05).The MDM2 protein positive rates in CIN and control groups were 14.9%(7/47) and 7.4%(4/54) respectively,but had no significant difference(P>0.05).However,no significant correlations were found between the infection rates of HPV6/11 and HPV16/18,as well as HPV infection and expression of P53,MDM2 proteins(P>0.05).Conclusions HPV infection is strongly related with CIN; P53 protein may partly participate the development and progression of CIN,whereas MDM2 protein may play a minor role.