绞股蓝种质资源遗传多样性及亲缘关系的ISSR分析

目的研究绞股蓝Gynostemma pentaphyllun种质资源的遗传多样性及亲缘关系。方法采用ISSR分子标记技术对来自全国12个省市不同生态环境的48份绞股蓝种质材料进行遗传多样性及亲缘关系分析。结果 100条ISSR引物中共筛选出15条扩增条带清晰、稳定性好且多态性明显的引物,48份材料DNA扩增共获得214个位点,其中多态位点206个,多态性比率(PPL)96.26%,平均每条引物扩增位点为14.27个;平均观察等位基因数(Na)、有效等位基因数(Ne)、Shannon多样性信息指数(I)和Nei’s基因多样性指数(H)分别为1.962 6、1.335 8、0.221 1和0.359 8;种质材料间的遗传相似系数变幅为0.57~0.96,平均为0.72。利用UPGMA聚类分析,以遗传相似系数0.71为界,48份材料聚为4大类。结论绞股蓝种质材料间的遗传多样性较高,种质间的亲缘关系与其地理分布和生态环境并不完全一致。

ISSR analysis on genetic diversities and phylogenetic relationships of Gynostemma pentaphyllun germplasm reseources

Objective To analyze the genetic diversities and phylogenetic relationships of Gynostemma pentaphyllun germplasm. Methods Genetic diversities and phylogenetic relationships of 48 G. pentaphyllun germplasm from 12 provinces in different ecological environments were analyzed by inter simple sequence repeat (ISSR) molecular makers. Results (1) Fifteen primers were selected from 100 primers which had clear and stable polymorphic bands. A total of 214 loci were obtained, including 206 loci, on average, amplification site of each primer 14.27 and polymorphic loci (PPL) was 96.26%. The averagely observed number of alleles (N_a), effective number of alleles (N_e), Shannon information index (I) and Nei's genetic index (H) were 1.962 6, 1.335 8, 0.221 1, and 0.359 8, respectively. The enetic similarity coefficient (GS) ranged from 0.57 to 0.96. Cluster annlysis with UPGMA method showed that the 48 testing materials were divided into four groups at the level GS 0.72. Conclusion The results of ISSR analysis reveal that G. pentaphyllun germplasm has a higher genetic diversity level and the genetic relationship is not entirely consistent with geographical distribution and ecological environment.