海洋细菌Bacillus sp.次生代谢产物的分离与鉴定

目的研究1株海洋细菌Bacillus sp.(No.DY1979)的次生代谢产物。方法采用各种色谱手段进行分离和纯化,根据各种光谱技术对单体化合物进行结构鉴定。结果分得12个化合物,分别鉴定为2,2′-二硫代二苯并噻唑(2,2′-dibenzothiazolyl disulfide,1)、2(3H)-苯并噻唑酮(2(3H)-benzothiazolone,2)、环(甘-脯)二肽(cyclo(Gly-Pro),3)、环(亮-脯)二肽(cyclo(Leu-Pro),4)、环(甘-丙)二肽(cyclo(Gly-Ala),5)、色氨酸(tryptophan,6)、1,2,3,4-四氢-3-羧基-2-卡波林(1,2,3,4-tetrahydro-3-carboxy-2-carboline,7)、胸腺嘧啶(thymine,8)、尿嘧啶(uracil,9)、苯乙酸(phenylace-tic acid,10)、邻苯二甲酸二异丁酯(1,2-benzenedicarboxylic acid diisobutyl ester,11)、邻苯二甲酸二正丁酯(1,2-benzenedicarboxylic acid dibutyl ester,12)。结论化合物1、2为含硫含氮的杂环类化合物,化合物1为细菌中首次分离,化合物2曾从另一株海洋细菌中分离得到;化合物3~9为含氮成分,其中化合物3~5为常见的细菌代谢产物环二肽,化合物6、7属氨基酸及其衍生物。

Isolation and identification of metabolites from a marine bacterial strain Bacillus sp.

Objective To study the metabolites of a marine bacterial strain Bacillus sp. (No. DY1979). Methods The compounds were isolated by chromatographic techniques and determined by spectroscopic methods. Results Twelve compounds were obtained and identified as 2,2'-dibenzothiazolyl disulfide (1), 2(3H)-benzothiazolone (2), cyclo(Gly-Pro) (3), cyclo(Leu-Pro) (4), cyclo(Gly-Ala) (5), tryptophan (6), 1,2,3,4-tetrahydro-3-carboxy-2-carboline (7), thymine (8), uracil (9), phenylacetic acid (10), 1,2-benzenedicarboxylic acid diisobutyl ester (11), and 1,2-benzenedicarboxylic acid dibutyl ester (12). Conclusions Compounds 1 and 2 are sulfur-containing and nitrogenous heterocycles: 2 is isolated from a marine bacterium obtained from tissues of a sponge before, whereas 1 is isolated from bacteria for the first time; Compounds 3-9 are nitrogenous compounds: 3-5 are cyclic dipeptides, whereas 6 and 7 are belong to amino acid and its derivative, respectively.