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| C6/SD大鼠胶质瘤模型的建立及C6胶质瘤细胞增殖动力学研究 | |
| 引用本文： | 罗林,司马秀田,白刚,赵建华,王廷华. C6/SD大鼠胶质瘤模型的建立及C6胶质瘤细胞增殖动力学研究[J]. 昆明医学院学报, 2006, 27(3): 31-34 |
| 作者姓名： | 罗林 司马秀田 白刚 赵建华 王廷华 |
| 作者单位： | 1. 昆明医学院第三附属医院神经外科,昆明,650118 2. 云南省第一人民医院神经外科,昆明,650032 3. 昆明医学院神经科学研究所,昆明,650031 |
| 摘 要： | 目的建立C6/SD大鼠脑胶质瘤模型,并在其基础上研究C6胶质瘤细胞的增殖动力学.方法应用免疫组化、HE染色和流式细胞仪测定,观察C6胶质瘤细胞的增殖动力学指标,并进行相关性分析.结果肿瘤细胞接种2周后,肿瘤模型建立,HE染色见肿瘤细胞增殖活跃,各项指标显示该肿瘤增殖活性较强. |
| 关 键 词： | C6胶质瘤细胞 增殖动力学 大鼠 |
| 文章编号： | 1003-4706(2006)03-0031-04 |
| 收稿时间： | 2006-04-03 |
| 修稿时间： | 2006-04-03 |
The Establishment of C6/SD Glioma Model And The Reseachment of Proliferation Kinetics in C6 Glioma Cell |
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| LUO Lin,SIMA Xiu-tian,BAI Gang,ZHAO Jian-hua,WANG Ting-hua. The Establishment of C6/SD Glioma Model And The Reseachment of Proliferation Kinetics in C6 Glioma Cell[J]. Journal of Kunming Medical College, 2006, 27(3): 31-34 | |
| Authors: | LUO Lin SIMA Xiu-tian BAI Gang ZHAO Jian-hua WANG Ting-hua |
| Affiliation: | 1 . Department of Neurosurgery, The 3rd Affiliated Hospial of Kunming Medical College, Kunming 650118 ; 2. The 1st People Hospial of Yunnan Provinces, Kunming 650032; 3. Institute of Neurosclence of Kunming Medical College, Kunming 650031, china |
| Abstract: | Objective To investigate the proliferation kinetics in C6 glioma cell in rat based on establishment of a C6/SD glioma model.Methods After establishing a C6/SD glioma model,by using flow cytometric analysis, immunohistochemistry and HE stain three methods, the proliferation kinetics in C6 glioma cell was measured, its dependablity analysis was carried out also.Results After two weeks infused C6 glioma cell, the rat glioma model was established, tumor cell proliferated activity in HE stain. All indicators show tumor cell had strong prolifercation activity. |
| Keywords: | C6 glioma cell proliferation kinetic SD rat |
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